| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
DNA methylation is associated with the inappropriate transcriptional silencing of cancer-related genes and affects the hallmarks of cancer such as sustaining proliferative signaling, resisting cell death, and activating invasion and metastasis. We found that genome-wide reactivation of hypermethylated genes suppressed growth of prostate cancer cell line LNCaP and induced apoptosis. We searched for apoptosis-inducing genes whose promoters were hypermethylated in prostate cancers. As a result, we found that the promoter region of PYCARD, one of apoptosis-inducing factor, were highly methylated in a cancer-specific manner (46/51: 90%). In addition, PYCARD expression induced apoptosis in LNCaP cells. These results suggest that aberrant promoter hypermethylation of PYCARD plays an important role in prostatic tumorigenesis.
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