An analyses of DMT1 Associated Protein that is related to celllular proliferation and iron metabolism
| Project/Area Number |
15K08398
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 鉄代謝 / DMT1 / DMT1 Associated Protein / 細胞増殖 |
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| Outline of Final Research Achievements |
Transport of iron is carried by divalent metal transporter 1(DMT1) in mammalian cell. In this grant, K562 was employed as an erythroid precursor model to study DMT1 Associated Protein(DAP). An analysis of DAP amino acid sequence revealed the binding domain to PBR. Protoporpyrin IX(PPIX) is presumed to bind with PBR, suggesting the interaction between DAP and PPIX via PBR. The expression levels of DAP, DMT1, transferrin receptor 1(TfR1), ferritin heavy chain(FTH) were examined after exposure of PPIX. PPIX decreased the protein level of DAP and DMT1 at 1 h. mRNA level of DAP was induced at 8 h and protein level was recovered at 24 h, while, mRNA of DMT1 was not increased until 24 h after PPIX exposure. C/EBPa, which transcribe DMT1 mRNA, were decreased by PPIX treatment. PPIX-induced degradation of DAP, FTH, DMT1 was presumed to be carried in lysosome and proteasome. The combination of lysosomal and proteasomal inhibitor were most effective way to prevent the degradation of FTH.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114)2017
Author(s)
Hirako, S., Tsuda, H., Ito, F., Okazaki, Y., Hirayama, T., Nagasawa, H., Nakano, T., Imai, K., Kotani, T,. F., Kikkawa, Toyokuni, S.
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 61
Issue: 3
Pages: 176-182
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed / Open Access
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[Journal Article] Non-Thermal Plasma Induces a Stress Response in Mesothelioma Cells Resulting in Increased Endocytosis, Lysosome Biogenesis and Autophagy.2017
Author(s)
Shi. L., Ito, F., Wang, Y., Okazaki, Y., Tanaka, H., Mizuno, M., Hori. M., Hirayama, T., Nagasawa, H., Richardson, Des R, Toyokuni, S.
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Journal Title
Free Radic. Biol. Med.
Volume: 108
Pages: 904-917
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Stapled BIG3 helical peptide ERAP potentiates antitumour activity for breast cancer therapeutics2017
Author(s)
Yoshimaru T, Aihara K, Komatsu M, Matsushita Y, Okazaki Y, Toyokuni S, Honda J, Sasa M, Miyoshi Y, Otaka A, Katagiri T
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Journal Title
Sci Rep.
Volume: in press
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Protein kinase A inhibition facilitates the antitumor activity of xanthohumol, a valosin-containing protein inhibitor2017
Author(s)
Y. Shikata Y, T. Yoshimaru, M. Komatsu, H. Katoh, R. Sato, S. Kanagaki, Y. Okazaki, S. Toyokuni, E. Tashiro, S. Ishikawa, T. Katagiri, M. Imoto,
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Journal Title
Cancer Science
Volume: 108
Issue: 4
Pages: 785-794
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.2016
Author(s)
Wang Y, Okazaki Y, Shi L, Kohda H, Tanaka M, Taki K, Nishioka T, Hirayama T, Nagasawa H, Yamashita Y, Toyokuni S.
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Journal Title
Cancer Sci
Volume: 107(3)
Issue: 3
Pages: 250-257
DOI
Related Report
Peer Reviewed / Open Access
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