| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Girdin play important roles for brain development at hippocampus, olfacrory blub/RMS, anterior commissure and GABAergic neurons of cortex. Girdin has two tyrosine phosphorylation sites and GEF domain, but these functions in vivo have not been clear.
To study the function of Girdin tyrosine phosphorylation at Y1764 and Y1798, we generated Girdin Y1764,1798F mutant mice. Mutant mice show no obvious phenotype at hippocampus and olfactory bulb/RMS, which may indicate the less important roles of Girdin tyrosine phosphorylation for cell motility at developing brain. In addition, we generate Girdin F1696A mutant mice, which would lack GEF activity of Girdin. Mutant mice show abnormal brain structure at hippocampus and olfactory blub/RMS resembling Girdin knockout mice. Mutant mice also show milder phenotypes at anterior commissure and distribution of GABAergic neurons in cortex. These data suggest importance of Girdin GEF activity for cell motility.
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