Overexpression of DDX27 in gastric cancer

• Project/Area Number: 15K08406
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Oita University
• Principal Investigator: Yoshiyuki Tsukamoto 大分大学, 医学部, 助教 (00433053)
• Co-Investigator(Kenkyū-buntansha): 守山 正胤 大分大学, 医学部, 教授 (90239707) ; 平下 有香 大分大学, 医学部, 医員 (70771955)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 胃癌 / DDX27 / シグナル伝達 / 分子標的 / 進行

Outline of Final Research Achievements

Previously, we reported that DDX27 gene is amplified and overexpressed with the progress of gastric carcinogenesis. In this project, we aimed to determine whether DDX27 contributes to malignancy of gastric cancer. Before we started this project, we found that expression of DDX27 is related to colony formation of gastric cancer cells and patients' survival. In this project, we analyzed the impact of DDX27 knockdown on gastric cancer cells and found that 1)DDX27 contributes to tumor formation in vivo, 2)DDX27 regulates cell cycle of gastric cancer cells and 3)DDX27 regulates phosphorylation of Akt. These results contribute to establish a novel molecular targeted therapy in gastric cancer. We reported these data in American Journal of Cancer Research.

Research Products

• [Int'l Joint Research] Clevers Group, Hubrecht Institute(オランダ)
• [Journal Article] Downregulation of dual-specificity phosphatase 4 enhances cell proliferation and invasiveness in colorectal carcinomas (2017)
  • Author(s): Ichimanda Michihiro、Hijiya Naoki、Tsukamoto Yoshiyuki、Uchida Tomohisa、Nakada Chisato、Akagi Tomonori、Etoh Tsuyoshi、Iha Hidekatsu、Inomata Masafumi、Takekawa Mutsuhiro、Moriyama Masatsugu
  • Journal Title: Cancer Sci Volume: 109 Issue: 1 Pages: 250-258
  • DOI: 10.1111/cas.13444
  • Peer Reviewed / Open Access
• [Journal Article] A 17-molecule set as a predictor of complete response to neoadjuvant chemotherapy with docetaxel, cisplatin, and 5-fluorouracil in esophageal cancer (2017)
  • Author(s): Fujishima Hajime、Fumoto Shoichi、Shibata Tomotaka、Nishiki Kohei、Tsukamoto Yoshiyuki、Etoh Tsuyoshi、Moriyama Masatsugu、Shiraishi Norio、Inomata Masafumi
  • Journal Title: PLoS One Volume: 12 Issue: 11 Pages: e0188098-e0188098
  • DOI: 10.1371/journal.pone.0188098
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of cannabinoid receptor 1 in esophageal squamous cell carcinoma is correlated with metastasis to lymph nodes and distant organs, and poor prognosis. (2017)
  • Author(s): Hijiya N, Shibata T, Daa T, Hamanaka R, Uchida T, Matsuura K, Tsukamoto Y, Nakada C, Iha H, Inomata M, Moriyama M.
  • Journal Title: Pathol Int. Volume: 67 Issue: 2 Pages: 83-90
  • DOI: 10.1111/pin.12495
  • Peer Reviewed / Open Access
• [Journal Article] Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells. (2016)
  • Author(s): Hirashita Y, Kodama M, Okimoto T, Murakami K, Moriyama M, et al.
  • Journal Title: Cancer Science Volume: 107(12) Issue: 12 Pages: 1919-1928
  • DOI: 10.1111/cas.13094
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genomic loss of DUSP4 contributes to the progression of intraepithelial neoplasm of pancreas to invasive carcinoma. (2016)
  • Author(s): Hijiya N, Tsukamoto Y, Nakada C, Tung NL, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.
  • Journal Title: Cancer Res. Volume: 76 Issue: 9 Pages: 2612-2625
  • DOI: 10.1158/0008-5472.can-15-1846
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway. (2016)
  • Author(s): Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
  • Journal Title: J Pathol. Volume: 印刷中 Issue: 1 Pages: 97-108
  • DOI: 10.1002/path.4706
  • Peer Reviewed / Open Access
• [Journal Article] Downregulation of WDR20 due to loss of 14q is involved in the malignant transformation of clear cell renal cell carcinoma. (2016)
  • Author(s): Takahashi M, Tsukamoto Y, Kai T, Tokunaga A, Nakada C, Hijiya N, Uchida T, Daa T, Nomura T, Sato F, Mimata H, Matsuura K, Moriyama M.
  • Journal Title: Cancer Science Volume: 107(4) Issue: 4 Pages: 417-423
  • DOI: 10.1111/cas.12892
  • Peer Reviewed / Open Access
• [Journal Article] Expression of DDX27 contributes to colony-forming ability of gastric cancer cells and correlates with poor prognosis in gastric cancer. (2015)
  • Author(s): Tsukamoto Y, Fumoto S, Noguchi T, Yanagihara K, Hirashita Y, Nakada C, Hijiya N, Uchida T, Matsuura K, Hamanaka R, Murakami K, Seto M, Inomata M, Moriyama M.
  • Journal Title: Am J Cancer Res Volume: 5(10) Pages: 2998-3014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Downregulation of NDUFB6 due to 9p24.1-p13.3 loss is implicated in metastatic clear cell renal cell carcinoma. (2015)
  • Author(s): Narimatsu T, *Matsuura K, Nakada C, Tsukamoto Y, Hijiya N, Kai T, Inoue T, Uchida T, Nomura T, Sato F, Seto M, Takeuchi I, Mimata H, Moriyama M
  • Journal Title: Cancer Medicine Volume: 4(1) Issue: 1 Pages: 112-124
  • DOI: 10.1002/cam4.351
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] 大分大学医学部分子病理学講座
  • URL: http://www.med.oita-u.ac.jp/byori2/index.htm