Development of novel form of mimic microRNA for microRNA replacement therapy.
| Project/Area Number |
15K08410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
熊谷 勝義 東京医科大学, 医学部, 助教 (20567911)
黒田 雅彦 東京医科大学, 医学部, 主任教授 (80251304)
原田 裕一郎 東京医科大学, 医学部, 助手 (80570168)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 核酸医薬 / microRNA / miR-34a / 肺がん / 炎症性サイトカイン |
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| Outline of Final Research Achievements |
The innate cytokine response to nucleic acid is the most challenging problem confronting the practical use of nucleic acid medicine. The degree of stimulation of the innate cytokine response strongly depends on the length of the nucleic acid. In this study, we developed a 30-nucleotide single strand RNA, termed "guide hairpin RNA (ghRNA, ghR)", that has a physiological function similar to that of miRNA and siRNA. The ghR caused no innate cytokine response either in vitro or in vivo. In addition, its structure does not contain a passenger strand seed sequence, reducing the unwanted gene repression relative to existing short RNA reagents. Systemic and local injection of ghR-form miR-34a (ghR-34a) suppressed tumor growth in a mouse model of RAS-induced lung cancer. This novel RNA interference (RNAi) technology may provide a novel, safe, and effective nucleic acid drug platform that will increase the clinical usefulness of nucleic acid therapy.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Development of novel small hairpin RNAs that do not require processing by dicer or AGO22016
Author(s)
Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Okuyama H, Kumagai K, Takanashi M, Sudo K, Ikeda N, Kuroda M.
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Journal Title
Mol Ther
Volume: 24
Issue: 7
Pages: 1278-1289
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Development of novel Dicer- and Ago2-independent small hairpin RNAs.2016
Author(s)
Shin-ichiro OHNO, Karen ITANO, Yuichirou HARADA, Koutaro ASADA, Keiki OIKAWA, Mikie KASHIWAZAKO, Hikaru OKUYAMA, Katsuyoshi KUMAGAI, Masakatsu TAKANASHI, Katsuko SUDO, Norihiko IKEDA, Masahiko KURODA.
Organizer
ASGCT 19th Annual Meeting 2016
Place of Presentation
Marriott Wardman Park, Washington, DC, USA.
Year and Date
2016-05-04
Related Report
Int'l Joint Research
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