Elucidating the role of long non-coding RNA in vivo by genome editing technologies
| Project/Area Number |
15K08421
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
CHIBA Tomoki 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (00645830)
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| Research Collaborator |
ASAHARA Hiroshi
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 長鎖非コードRNA / 炎症性サイトカイン / ゲノム編集 / 非コードRNA / 炎症性腸炎 / 炎症 / サイトカイン |
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| Outline of Final Research Achievements |
Recent advantages of transcriptome analyses revealed that non-coding RNAs, especially long non-coding (lnc) RNAs, are transcribed across genome and involved in various aspects of biological processes. I have identified a novel lncRNA that positively regulates inflammatory cytokine expression and named LASC. LASC is required for the expression of inflammatory cytokines such as IL-6 and GM-CSF. Recruitment of transcription factor NF-kB was strongly reduced in LASC deficient cells, suggesting that LASC could regulate inflammatory cytokine expression at transcriptional level. In order to clarify the role of LASC in inflammation in vivo, LASC knockout mice were generated by genome editing technology and showed resistance to endotoxin shock.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトやマウスにおいて、タンパク質をコードしない非コードRNA（non-coding RNA）は20,000種類以上あると推定されている。その多くは機能を持たないRNAであると考えられているが、一部のRNAは生命機能に重要な役割を果たしている。LASCは炎症性サイトカインの発現に重要な長鎖非コードRNAであり、個体における炎症応答に重要なRNAであることが示された。
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] Ablation of miR-146b in mice causes hematopoietic malignancy2018
Author(s)
Mitsumura Takahiro、Ito Yoshiaki、Chiba Tomoki、Matsushima Takahide、Kurimoto Ryota、Tanaka Yoko、Kato Tomomi、Uchida Keisuke、Ito Takashi、Yamamoto Kouhei、Eishi Yoshinobu、Kitagawa Masanobu、Miyazaki Yasunari、Inase Naohiko、Asahara Hiroshi
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Journal Title
Blood Advances
Volume: 2
Issue: 23
Pages: 3483-3491
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproductiontran .2017
Author(s)
Nakasuji T, Ogonuki N, Chiba T, Kato T, Shiozawa K, Yamatoya K, Tanaka H, Kondo T, Miyado K, Miyasaka N, Kubota T, Ogura A, Asahara H*.
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Journal Title
PLoS Genetics.
Volume: 13
Issue: 1
Pages: e1006578-e1006578
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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