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Analysis of Regulatory Mechanism of RANK Gene Expression

Research Project

Project/Area Number 15K08426
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionEhime University

Principal Investigator

KITAZWA RIKO  愛媛大学, 医学部附属病院, 准教授 (00273780)

Co-Investigator(Kenkyū-buntansha) 原口 竜摩  愛媛大学, 医学系研究科, 講師 (00423690)
水野 洋輔  愛媛大学, 医学部附属病院, 助教 (90748021)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords破骨細胞 / RANK / 遺伝子プロモータ / 遺伝子改変動物 / メチル化 / 転写因子
Outline of Final Research Achievements

Receptor activator of NF-kB (RANK) is a member of the TNF receptor family expressed in osteoclast precursors, and RANK-RANK ligand signaling is essential for osteoclastogenesis. We have identified a novel alternative splicing variant of RANK gene (vRANK) encoding the short molecule. When overexpressed in vitro, vRANK in RAW264.7 cells decreased the formation of osteoclasts. When systemically overexpressed in vivo, the CAGcre+/vRANK mouse showed high fatality. When selectively overexpressed among the monocyte-macrophage lineage, the LysMcre+/vRANK mouse showed decreased osteoclastogenesis in ex vivo culture of spleen cells, and increased bone mass measured by micro CT. These results suggest that vRANK is a novel bioactive peptide that might educes the number of osteoclasts

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015

All Journal Article (14 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 12 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (15 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Novel GLI3 variant causing overlapped Greig cephalopolysyndactyly syndrome (GCPS) and Pallister-Hall syndrome (PHS) phenotype with agenesis of gallbladder and pancreas.2018

    • Author(s)
      Ito S, Kitazawa R, Haraguchi R, Kondo T, Ouchi A, Ueda Y, Kitazawa S.
    • Journal Title

      Diagn Pathol.

      Volume: 3;13(1):1 Issue: 1 Pages: 1-4

    • DOI

      10.1186/s13000-017-0682-8

    • NAID

      120006425668

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathologic conditions of hard tissue: role of osteoclasts in osteolytic lesion.2018

    • Author(s)
      Kitazawa R, Haraguchi R, Fukushima M, Kitazawa S.
    • Journal Title

      Histochem Cell Biol

      Volume: 149(4) Issue: 4 Pages: 405-415

    • DOI

      10.1007/s00418-018-1639-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone.2018

    • Author(s)
      Haraguchi R, Kitazawa R, Imai Y, Kitazawa S.
    • Journal Title

      Histochem Cell Biol

      Volume: 149(4) Issue: 4 Pages: 365-373

    • DOI

      10.1007/s00418-018-1641-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] the posterior cranial fossa: Pediatric medulloepithelioma diagnosis2017

    • Author(s)
      Kosuke Kusakabe, Shohei Kohno, Akihiro Inoue, Riko Kitazawa, Takeharu KuniedaTakeharu Kunieda
    • Journal Title

      Neuropathology

      Volume: 38 Issue: 2 Pages: 179-184

    • DOI

      10.1111/neup.12431

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] sFRP4-dependent Wnt signal modulation is critical for bone remodeling during postnatal development and age-related bone loss.2016

    • Author(s)
      Haraguchi R, Kitazawa R, Mori K, Tachibana R, Kiyonari H, Imai Y, Abe T, Kitazawa S.
    • Journal Title

      Sci Rep.

      Volume: 6:25198 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep25198

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TDRG1 functions in testicular seminoma are dependent on the PI3K/Akt/mTOR signaling pathway2016

    • Author(s)
      Yong Wang, Yu Gan, Zhengyu Tan, Jun Zhou, Riko Kitazawa, Xianzhen Jiang, Yuxin Tang, and Jianfu Yang
    • Journal Title

      OncoTargets and Therapy

      Volume: 7 Issue: 7 Pages: 409-420

    • DOI

      10.1080/15384047.2016.1178425

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inflammatory Fibroid Polyps of Large Bowel with PDGFRA Mutation2016

    • Author(s)
      Kanazu Ariyasu, Riko Kitazawa, Ryuma Haraguchi, Yasuo Ueda, Yukino Kawanami, Yukiko Nishi, Yuri Kameoka, Yosuke Mizuno and Sohei Kitazawa
    • Journal Title

      J Cell Sci Ther

      Volume: 7 Issue: 01 Pages: 234-239

    • DOI

      10.4172/2157-7013.1000234

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hepatic Sinusoidal Obstruction Syndrome without Preceding Medical Events.2016

    • Author(s)
      Yukino Kawanami, Riko Kitazawa, Ryuma Haraguchi, Yasuo Ueda, Yukiko Nishi, Kanazu Ariyasu, Yosuke Mizuno, Sohei Kitazawa
    • Journal Title

      Case Reports in Clinical Medicine

      Volume: 5 Issue: 03 Pages: 105-108

    • DOI

      10.4236/crcm.2016.53019

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CPC解説(第80回) 急速な経過をたどった重症熱性血小板減少症候群(SFTS)の一剖検例.2016

    • Author(s)
      水野洋輔, 北澤 理子
    • Journal Title

      病理と臨床

      Volume: 34 Pages: 297-301

    • Related Report
      2016 Research-status Report
  • [Journal Article] Epigenetic regulation of Tbx18 gene expression during endochondral bone formation.2015

    • Author(s)
      Haraguchi R, Kitazawa R, Kitazawa S
    • Journal Title

      Cell Tissue Res

      Volume: 359 Issue: 2 Pages: 503-512

    • DOI

      10.1007/s00441-014-2028-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reactivation of CDX2 in Gastric Cancer as Mark for Gene Silencing Memory2015

    • Author(s)
      Kameoka Y, Kitazawa R, Ariasu K, Tachibana R, Mizuno Y, Haraguchi R, Kitazawa S.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 48 Issue: 4 Pages: 115-124

    • DOI

      10.1267/ahc.15014

    • NAID

      130005095243

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Parathyroid Hormone-related Peptide-producing Multiple Myeloma and Renal Impairment2015

    • Author(s)
      Masaru Kinomura, Noriaki Shimada, Mana Nishikawa, Kazuyoshi Omori, Tomoyasu Jo, Yasunori Ueda, Kenji Notohara, Riko Kitazawa, Sohei Kitazawa, Masaki Fukushima, and Kenichiro Asanoa
    • Journal Title

      Internal Medicine

      Volume: 54 Issue: 23 Pages: 3029-3033

    • DOI

      10.2169/internalmedicine.54.5085

    • NAID

      130005112841

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 骨巨細胞腫の原因遺伝子と抗RANKL抗体を用いた治療法の開発2015

    • Author(s)
      北澤荘平、北澤理子
    • Journal Title

      病理と臨床

      Volume: 33 Pages: 1021-1023

    • Related Report
      2015 Research-status Report
  • [Journal Article] 6Two cases of pineal-region meningiomas derived from arachnoid membrane over the vein of Galen without dural attachment.2015

    • Author(s)
      Akihiro Inoue, Takanori Ohnishi, Shohei Kohno, Yoshihiro Ohtsuka, Yawara Nakamura, Yosuke Mizuno, Riko Kitazawa and Shiro Ohue
    • Journal Title

      World Journal of Surgical Oncology

      Volume: 13 Issue: 1 Pages: 226-226

    • DOI

      10.1186/s12957-015-0645-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの機能解析2017

    • Author(s)
      北澤 理子, 原口 竜摩, 小林 泰浩, 北澤 荘平
    • Organizer
      日本骨代謝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 長管骨伸長時におけるヘッジホッグシグナル受容細胞の組織系譜解析2017

    • Author(s)
      原口 竜摩, 北澤 理子, 今井 祐記, 北澤 荘平
    • Organizer
      日本骨代謝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 下部消化管糖尿病合併症におけるヘッジホッグシグナル経路の関与2017

    • Author(s)
      倉田菜央、原口 竜摩, 北澤 理子, 北澤荘平
    • Organizer
      日本組織細胞化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 糖尿病による精子形成能低下とヘッジホッグシグナル経路の関連性について2017

    • Author(s)
      大野 輝之, 原口 竜摩, 齋藤 洋太, 下山 貴幸, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2017

    • Author(s)
      北澤理子、北澤荘平ほか
    • Organizer
      日本病理学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Function of novel splicing variant of NF-kB receptor activator (vRANK)2016

    • Author(s)
      Kitazawa R, Haraguchi R, Mizuno Y, Kobayashi K, Kitazawa S
    • Organizer
      ASBMR 2016
    • Place of Presentation
      Atlanta, Georgia, USA
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional role of Hedgehog signaling in osteoclast lineage2016

    • Author(s)
      Haraguchi R, Imai Y, Kitazawa R, Kitazawa S
    • Organizer
      ASBMR 2016
    • Place of Presentation
      Atlanta, Georgia, USA
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2016

    • Author(s)
      北澤理子、原口竜摩、水野洋輔、上田康雄、小林泰浩、北澤荘平
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析 Identification and analysis of function of a novel splicing variant of receptor activation of NF-kB2016

    • Author(s)
      Kitazawa R, Haraguchi R, Ueda Y, Mizuno Y, Kitazawa S
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-05-12
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス前破骨細胞株RAW264 細胞における受容体RANK遺伝子発現調節領域のメチル化と破骨細胞分化能の解析2016

    • Author(s)
      村田夕紀、北澤理子、原口竜摩、北澤荘平
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-05-12
    • Related Report
      2016 Research-status Report
  • [Presentation] Function of novel splicing variant of receptor activator of NF-κB.2015

    • Author(s)
      Kitazawa R, Haraguchi R, Mizuno Y, Kobayashi Y. Kitazawa S.
    • Organizer
      ASBMR 2015
    • Place of Presentation
      Seattle Convention Center, Seattle, WA, USA
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The role of Wnt signal modulator, sFRP4, in bone formation and metabolism.2015

    • Author(s)
      Haraguchi R, Kitazawa R, Mizuno Y, Kobayashi Y. Kitazawa S.
    • Organizer
      ASBMR 2015
    • Place of Presentation
      Seattle Convention Center, , WA, USA
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2015

    • Author(s)
      北澤 理子, 原口 竜摩, 水野 洋輔, 小林 泰浩, 北澤 荘平
    • Organizer
      第33回日本骨代謝学会学術総会
    • Place of Presentation
      京王プラザホテル. 東京都新宿区
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2015

    • Author(s)
      北澤 理子, 原口 竜摩, 水野 洋輔, 北澤 荘平
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場, 名古屋市
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス前破骨細胞株RAW264細胞における受容体RANK遺伝子発現調節領域のメチル化と破骨細胞分化能の解析2015

    • Author(s)
      村田 夕紀, 北澤 荘平, 原口 竜摩, 北澤 理子.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場, 名古屋市
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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