| Project/Area Number |
15K08428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
ARAKAWA Takeshi 琉球大学, 熱帯生物圏研究センター, 教授 (50305190)
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| Co-Investigator(Kenkyū-buntansha) |
原國 哲也 琉球大学, 熱帯生物圏研究センター, 協力研究員 (60593598)
玉城 志博 琉球大学, 熱帯生物圏研究センター, 助教 (00720822)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 感染症 / ワクチン / 組換えタンパク質発現 / タンパク質リフォールディング / 腸管毒素 / 志賀毒素 / 大腸菌 / 食中毒 / 腸管毒素原性大腸菌 / 易熱性腸管毒素 / 耐熱性腸管毒素 / コレラ毒素 / ジスルフィド結合 / 天然型タンパク質立体構造 / 腸管毒素原性大腸菌（ETEC） / 易熱性腸管毒素（LT） / 耐熱性腸管毒素（STa） / サブユニットワクチン / コレラ毒素（CT） / 交叉反応性 / ５量体 |
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| Outline of Final Research Achievements |
In this study, we developed a new protein expression and refolding method to produce B subunit pentamer molecules derived from enterotoxins such as cholera toxin (CT), heat-labile enterotoxin (LT) and Shiga toxin (Stx). Those B subunits are to be included in recombinant vaccines to combat against enterotoxin-producing pahthogenic Escherichia coli. Our techniques are considered to be uniuqe in that pentameric B subunit proteins are highly uniform in a molecular structure, although they are often produced as high-molecular mass protein aggregates when refolded from inclustion bodies of E. coli.
These findings would be valuable to design molecular vaccines against enteropathogenic E. coli producing LT (enterotoxigenic E. coli: ETEC) or Stx (enterohemorrhagic E. coli: EHEC).
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