Molecular mechanism of organ fibrosis and vascular calcification due to metabolic abnormality of phosphate and calcium
| Project/Area Number |
15K08430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Research Collaborator |
Wei Ran
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 高リン血症 / 血管石灰化 / 酸化ストレス / 血管平滑筋細胞 / 石灰化 / オートファジー |
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| Outline of Final Research Achievements |
Alteration of phosphate and calcium in the body, especially high phosphate and/or high calcium is closely related to vascular calcification. We investigated the molecular mechanism by which hyperphosphatemia or hypercalcemia causes vascular calcification, focusing on the association between oxidative stress and vascular calcification. We used cultured vascular smooth muscle cells (VSMCs) to examine calcification when they were cultured in the media with high phosphate. When the cultured VSMCs were cultured for 7 days in the medium with high phosphate, calcified materials were precipitated in and out of the cells. At the same time, we found that intracellular oxidative stress signals Keap1/ Nrf2 were altered, indicating that oxidative stress is involved in the mechanism of vascular smooth muscle cell calcification. In addition, we examined the interrelationship between oxidative stress and autophagy, by which gets rid of calcification materials precipitated in the cells.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Impact of heart-specific disruption of the circadian clock on systemic glucose metabolism in mice2017
Author(s)
Nakao T, Kohsaka A, Otsuka T, Thein ZL, Le HT, Waki H, Gouraud SS, Ihara H, Nakanishi M, Sato F, Muragaki Y, Maeda M.
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Journal Title
Chronobiol Int
Volume: in press
Issue: 4
Pages: 499-510
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Refractory Ascites with Liver fibrosis developed in late phase allogeneic hematopoietic stem cell transplantation: report of three patients2016
Author(s)
Hosoi H, Warigaya K, Murata S, Mushino T, Kuriyama K, Nishikawa A, Tamura S, Hatanaka K, Hanaoka N, Muragaki Y, Murata S, Nakakuma H, Sonoki T.
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Journal Title
Hamatol Rep
Volume: 8
Issue: 2
Pages: 6482-6482
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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