| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Alteration of phosphate and calcium in the body, especially high phosphate and/or high calcium is closely related to vascular calcification. We investigated the molecular mechanism by which hyperphosphatemia or hypercalcemia causes vascular calcification, focusing on the association between oxidative stress and vascular calcification. We used cultured vascular smooth muscle cells (VSMCs) to examine calcification when they were cultured in the media with high phosphate. When the cultured VSMCs were cultured for 7 days in the medium with high phosphate, calcified materials were precipitated in and out of the cells. At the same time, we found that intracellular oxidative stress signals Keap1/ Nrf2 were altered, indicating that oxidative stress is involved in the mechanism of vascular smooth muscle cell calcification. In addition, we examined the interrelationship between oxidative stress and autophagy, by which gets rid of calcification materials precipitated in the cells.
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