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Elucidation of the mechanisms of carcinogenesis regulated by oxidative stress response

Research Project

Project/Area Number 15K08438
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionChiba Cancer Center (Research Institute)

Principal Investigator

WAKABAYASHI Yuichi  千葉県がんセンター(研究所), 発がん研究グループ 実験動物研究室, 室長 (40303119)

Co-Investigator(Kenkyū-buntansha) 磯貝 恵理子  千葉県がんセンター(研究所), 発がん研究グループ 実験動物研究室, 上席研究員 (40300917)
Co-Investigator(Renkei-kenkyūsha) SAKAKIBARA Yasubumi  慶応義塾大学, 理工学部, 教授 (10287427)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsMeis1 / Stk25 / 皮膚発がん / 化学発がん / ChIP sequencing / SU11274 / DMBA / TPA / 扁平上皮がん / Hif1α / p16 / p19 / Chipシーケンシング
Outline of Final Research Achievements

Meis1 has been shown to play an important role in leukemogenesis. However, its functions in skin carcinogenesis remain poorly understood. The c-Met inhibitor SU11274 has been identified through drug screening with HOXA9/Meis1-induced AML cell lines. We treated Meis1 knockdown cells with SU11274. As a result, SU11274 suppressed cell proliferation only in the presence of Meis1. In addition, to investigate the downstream genes of Meis1, we carried out ChIP sequencing analysis using chemically induced skin tumors. We carried out the luciferase assay with the promoter region of Stk25, one of Meis1 downstream candidate genes. As a result, strong luciferase activity was detected when B9 cells were transfected with the luciferase construct and full length Meis1 vector. We then generated Stk25 knockdown cell lines and did the invasion assay. As a result, Stk25 knockdown cells showed the reduction of the invasion activity as Meis1 knock down cell lines did.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (32 results)

All 2017 2016 2015 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (24 results) (of which Int'l Joint Research: 12 results) Remarks (2 results)

  • [Journal Article] The parathyroid hormone regulates skin tumour susceptibility in mice2017

    • Author(s)
      Okumura Kazuhiro、Saito Megumi、Yoshizawa Yasuhiro、Munakata Haruka、Isogai Eriko、Miura Ikuo、Wakana Shigeharu、Yamaguchi Midori、Shitara Hiroshi、Taya Choji、Karaplis Andrew C.、Kominami Ryo、Wakabayashi Yuichi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 11208-11208

    • DOI

      10.1038/s41598-017-11561-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CENP-R acts bilaterally as a tumor suppressor and as an oncogene in the two-stage skin carcinogenesis model2017

    • Author(s)
      Okumura Kazuhiro、Kagawa Naoko、Saito Megumi、Yoshizawa Yasuhiro、Munakata Haruka、Isogai Eriko、Fukagawa Tatsuo、Wakabayashi Yuichi
    • Journal Title

      Cancer Sci.

      Volume: 108 Issue: 11 Pages: 2142-2148

    • DOI

      10.1111/cas.13348

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DEclust: A statistical approach for obtaining differential expression profiles of multiple conditions.2017

    • Author(s)
      Aoto Y, Hachiya T, Okumura K, Hase S, Sato K, Wakabayashi Y, and Sakakibara Y.
    • Journal Title

      PLoS One

      Volume: 12 (11) Issue: 11 Pages: e0188285-e0188285

    • DOI

      10.1371/journal.pone.0188285

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meis1 is required for c-Met inhibition to suppress cell proliferation of skin squamous cell carcinoma cells.2016

    • Author(s)
      M.Saito, K.Okumura, Y.Yoshizawa, H.Munakata, E.Isogai, Y.Wakabayashi
    • Journal Title

      J Biosci Med.

      Volume: 4 Issue: 07 Pages: 53-65

    • DOI

      10.4236/jbm.2016.47007

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A 1-bp deletion in Fgf5 causes male-dominant long hair in the Syrian hamster.2015

    • Author(s)
      Yoshizawa Y, Wada K, Shiomi G, Kameyama Y, Wakabayashi Y, Fukuta K, and Hashizume R.
    • Journal Title

      Mamm Genome.

      Volume: 26 Issue: 11-12 Pages: 630-637

    • DOI

      10.1007/s00335-015-9608-5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oncogenic <i>Lmo3</i> cooperates with <i>Hen2</i> to induce hydrocephalus in mice2015

    • Author(s)
      Isogai E, Okumura K ,Saito M, Yoshizawa Y, Itoh K, Tando S, Ohira M, Haraguchi S, Nakagawara A, Fushiki S, Nagase H, Wakabayashi Y
    • Journal Title

      Experimental Animals

      Volume: 64 Issue: 4 Pages: 407-414

    • DOI

      10.1538/expanim.15-0026

    • NAID

      130005104844

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒストンシャペロンHIRAによるストレス応答とがん悪性化の制御2017

    • Author(s)
      長垣良和、奥村和弘、小櫻祐司、高野洋志、中戸隆一郎、白髭克彦、八尾良司、若林雄一、石川冬木
    • Organizer
      平成28年度先端モデル動物支援プラットフォーム成果発表会
    • Place of Presentation
      琵琶湖ホテル
    • Year and Date
      2017-02-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 副甲状腺ホルモンPthはマウス皮膚腫瘍形成を抑制する2017

    • Author(s)
      奥村和弘、斎藤慈、吉澤康博、宗形春花、磯貝恵理子、三浦 郁夫、若菜茂晴、島貫碧、設楽浩志、多屋長治、木南凌、若林雄一
    • Organizer
      平成28年度先端モデル動物支援プラットフォーム成果発表会
    • Place of Presentation
      琵琶湖ホテル
    • Year and Date
      2017-02-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 副甲状腺ホルモンは皮膚腫瘍形成を制御する2017

    • Author(s)
      奥村和弘、斎藤慈、吉澤康博、宗形春花、磯貝恵理子、三浦 郁夫、若菜茂晴、島貫碧、設楽浩志、多屋長治、木南凌、若林雄一
    • Organizer
      第30回モロシヌス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 発がん抵抗性遺伝子座Stmm3の原因遺伝子の同定と機能解析2017

    • Author(s)
      斎藤慈、奥村和弘、吉澤康博、宗形春花、磯貝恵理子、若林雄一
    • Organizer
      第30回モロシヌス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 化学発がんにより誘発した皮膚腫瘍におけるMeis1の転写制御2017

    • Author(s)
      吉澤康博、奥村和弘、斎藤慈、宗形春花、青戸良賢、磯貝恵理子、榊原康文、若林雄一
    • Organizer
      第30回モロシヌス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 発がん抵抗性遺伝子座Stmm3の原因遺伝子の同定と機能解析2017

    • Author(s)
      斎藤慈、奥村和弘、吉澤康博、宗形春花、磯貝恵理子、若林雄一
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 副甲状腺ホルモンは表皮細胞内カルシウムを制御し皮膚腫瘍形成を抑制する2017

    • Author(s)
      奥村和弘、斎藤慈、、吉澤康博、宗形春花、磯貝恵理子、木南凌、若林雄一
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経芽腫関連遺伝子Lmo3とHen2は、協調的に機能してマウスにおける水頭症の発症に関与している2016

    • Author(s)
      磯貝恵理子、奥村和弘、斎藤慈、吉澤康博、宗形春花、伊藤恭子、丹藤創、大平美紀、原口清輝、中川原章、伏木信次、永瀬浩喜、若林雄一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス扁平上皮がん由来細胞を用いたc-Met阻害におけるMeis1の機能解析2016

    • Author(s)
      斎藤慈、奥村和弘、吉澤康博、宗形春花、磯貝恵理子、若林雄一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多段階皮膚発がん実験を用いたマウスがん抵抗性遺伝子座 Stmm1aの遺伝学的解析2016

    • Author(s)
      奥村和弘、吉澤康博、斎藤慈、、宗形春花、磯貝恵理子、三浦 郁夫、若菜茂晴、木南 凌、若林雄一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 皮膚悪性腫瘍においてMeis1が制御する下流因子の探索2016

    • Author(s)
      吉澤康博、奥村和弘、斎藤慈、宗形春花、青戸良賢、磯貝恵理子、榊原康文、若林雄一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ChIP sequencingにより示唆されたMeis1の皮膚腫瘍におけるグルコース代謝の制御2016

    • Author(s)
      吉澤康博、奥村和弘、斎藤慈、宗形春花、青戸良賢、磯貝恵理子、榊原康文、若林雄一
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス4番染色体上に存在する腫瘍悪性化を制御する原因遺伝子の探索2016

    • Author(s)
      斎藤慈、奥村和弘、吉澤康博、宗形春花、磯貝恵理子、若林雄一
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Stmm1の遺伝的解析と候補遺伝子の探索2016

    • Author(s)
      奥村和弘、斎藤慈、、吉澤康博、宗形春花、磯貝恵理子、木南凌、若林雄一
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 副甲状腺ホルモンPthはマウス皮膚腫瘍形成を抑制する2016

    • Author(s)
      奥村和弘、斎藤慈、吉澤康博、宗形春花、磯貝恵理子、三浦郁夫、若菜茂晴、島貫碧、設楽浩志、多屋長治、木南凌、若林雄一
    • Organizer
      第31回発癌病理研究会
    • Place of Presentation
      信州松代ロイヤルホテル
    • Year and Date
      2016-08-23
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス4番染色体上に存在する腫瘍悪性化を制御する原因遺伝子の探索2016

    • Author(s)
      斎藤慈、奥村和弘、吉澤康博、宗形春花、磯貝恵理子、若林雄一
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ChIP sequencingをもちいた皮膚腫瘍におけるMeis1の機能解析2016

    • Author(s)
      吉澤康博、奥村和弘、斎藤慈、宗形春花、青戸良賢、磯貝恵理子、榊原康文、若林雄一
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] コンジェニックマッピングおよび遺伝子発現解析による皮膚がん抵抗性遺伝子座Stmm1aの原因遺伝子探索2016

    • Author(s)
      奥村和弘、斎藤慈、吉澤康博、宗形春花、磯貝恵理子、三浦 郁夫、若菜茂晴、木南凌、若林雄一
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A cancer modifier role for Parathyroid Hormone in mouse skin carcinogenesis2015

    • Author(s)
      Kazuhiro Okumura, Megumi Saito, Yasuhiro Yoshizawa, Eriko Isogai, Yuichi Wakabayashi
    • Organizer
      IMGC 2015 29th International Mammalian Genome Conference
    • Place of Presentation
      横浜開港記念会館
    • Year and Date
      2015-11-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genetic analysis of Stmm3 locus controling tumor progression in a Japanese wild-derived mouse strain, MSM/Ms2015

    • Author(s)
      Megumi Saito, Kazuhiro Okumura, Yasuhiro Yoshizawa, Eriko Isogai, Yuichi Wakabayashi
    • Organizer
      IMGC 2015 29th International Mammalian Genome Conference
    • Place of Presentation
      横浜開港記念会館
    • Year and Date
      2015-11-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス皮膚発がんにおける副甲状腺ホルモンの機能解析2015

    • Author(s)
      奥村和弘,齋藤慈,吉澤康博,磯貝恵理子,木南凌,若林雄一
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 腫瘍悪性化を制御する Stmm3遺伝子座の遺伝学的解析2015

    • Author(s)
      齋藤慈,奥村和弘,磯貝恵理子,若菜茂晴,木南凌,若林雄一
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 発がん抵抗性遺伝子座Stmm3の遺伝学的解析2015

    • Author(s)
      斎藤慈、奥村和弘、磯貝恵理子、三浦郁生、若菜茂晴、木南凌、若林雄一
    • Organizer
      第62回日本実験動物学会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 副甲状腺ホルモンのマウス皮膚腫瘍形成における機能2015

    • Author(s)
      奥村和弘、斎藤慈、磯貝恵理子、三浦郁生、若菜茂晴、島貫碧、設楽浩志、多屋長治、木南凌、若林雄一
    • Organizer
      第62回日本実験動物学会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Remarks] 千葉県がんセンター研究所実験動物研究室

    • URL

      https://www.pref.chiba.lg.jp/gan/kenkyujo/soshiki/jikkendobutsu/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 千葉県がんセンター研究所実験動物研究室

    • URL

      http://www.pref.chiba.lg.jp/gan/kenkyujo/soshiki/jikkendobutsu/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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