| Project/Area Number |
15K08444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川合 覚 獨協医科大学, 医学部, 教授 (70275733)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Osamu 長崎大学, 熱帯医学研究所, 教授 (50325370)
MURATA Kazuyoshi 生理学研究所, 脳機能計測・支援センター, 准教授 (20311201)
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | サルマラリア / 感染赤血球 / 接着現象 / HUVEC / 電子顕微鏡 |
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| Outline of Final Research Achievements |
Electron microscopy demonstrated that the ultrastructure of P. knowlesi-infected human red blood cell (RBC) was similar to that of monkey red blood cell, and there was no structural difference between the iRBCs.
Next P. knowlesi-infected monkey RBCs were panned with Human Umbilical Vein Endothelial Cells (HUVECs), and cytoadherent P. knowlesi parasites were obtained. Transcriptome analysis of the obtained parasite line with high binding activity and non-binding parasites clearly indicated that two Schizont Infected Cell Agglutination (SICA) variant protein fragments were specifically and highly expressed in the binding parasite population only, whereas no other molecules were differentially transcribed.
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