Analysis for expression of miRNA in the patients with Schistosomiasis liver fibrosis.

• Project/Area Number: 15K08445
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: KIKUCHI Mihoko 長崎大学, 熱帯医学研究所, 講師 (40336186)
• Co-Investigator(Kenkyū-buntansha): 濱野 真二郎 長崎大学, 熱帯医学研究所, 教授 (70294915)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 住血吸虫症 / 肝線維化症 / マウスモデル / バイオマーカー / miRNA / マウス / マイクロRNA / マウス肝線維化症モデル / マンソン住血吸虫 / 日本住血吸虫 / 動物モデル

Outline of Final Research Achievements

Schistosomiasis is a parasitic disease caused by blood flukes (trematodes), including species of Schitosomiasis japonicum, mekongi and mansoni were parasited in portal vein and mesentery vein system. The big difference is admitted that the clinical condition of liver fibrosis by 3 kinds of this schistosome infection, but the true condition doesn't become clear. There are no effective treatments to liver fibrosis except to inhibit to infection in the present state. It is necessary for early diagnosis of liver fibrosis and need to perform Praziquantel treatment immediately, although tool of diagnose is established yet. So the clinical condition analysis using infection of 3 kinds of schistosome by a mouse model, I aimed at manifested profiling of microRNA of an endoplasmic reticulum in the serum, did a comparative analysis and tried explication of the different clinical condition as well as considered a possibility as the biomarker that a clinical condition change is detected.

Research Products

• [Journal Article] Schistosoma mansoni infection suppresses the growth of Plasmodium yoelii parasites in the liver and reduces gametocyte infectivity to mosquitoes. (2018)
  • Author(s): Moriyasu, T., Nakamura, R., Deloer, S., Senba, M., Kubo, M., Inoue, M., Culleton, R., Hamano, S.
  • Journal Title: PLoS Negl. Trop. Dis. e0006197. Volume: 12 Issue: 1 Pages: e0006197-e0006197
  • DOI: 10.1371/journal.pntd.0006197
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Intestinal Inflammation-Mediated Clearance of Amebic Parasites Is Dependent on IFN-γ (2018)
  • Author(s): Shimokawa C, Senba M, Kobayashi S, Kikuchi M, Obi S, Olia A, Hamano S, Hisaeda H
  • Journal Title: Journal of Immunology Volume: 200 Issue: 3 Pages: 1101-1109
  • DOI: 10.4049/jimmunol.1700806
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of Schistosoma japonicum CP1412 protein as a novel member of the ribonuclease T2 molecule family with immune regulatory function. (2017)
  • Author(s): Ke XD, Shen S, Song LJ, Yu CX, Kikuchi M, Hirayama K, Gao H, Wang J, Yin X, Yao Y, Liu Q, Zhou W.
  • Journal Title: Parasite and Vectors. Volume: 10(1) Issue: 1 Pages: 89-89
  • DOI: 10.1186/s13071-016-1962-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatial distribution and risk factors of Schistosoma haematobium and hookworm infections among schoolchildren in Kwale, Kenya. (2017)
  • Author(s): Chadeka EA, Nagi S, Sunahara T, Cheruiyot NB, Bahati F, Ozeki Y, Inoue M, Osada-Oka M, Okabe M, Hirayama Y, Changoma M, Adachi K, Mwende F, Kikuchi M, Nakamura R, Kalenda YDJ, Kaneko S, Hirayama K, Shimada M, Ichinose Y, Njenga SM, Matsumoto S, Hamano S.
  • Journal Title: PLoS Negl Trop Dis Volume: 11 Issue: 9 Pages: e0005872-e0005872
  • DOI: 10.1371/journal.pntd.0005872
  • NAID: 120006987400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Schistosomiasis in the Philippines: Challenges and some successes in Control. (2016)
  • Author(s): Lydia Leonardo, Yuichi Chigusa, Mihoko Kikuchi, Naoko Kato-Hayashi, Shin-ichiro Kawazu, Jose Ma Angeles, Ian Kendrich Fontanilla, Ian Kim Tabios, Kharleezelle Moendeg, Yasuyuki Goto, Raffy Jay Fornillos, Pebbles Grayle Tamayo and James Christoper Chua.
  • Journal Title: Southeast Asian journal of tropical medicine and public health Volume: 47(4) Pages: 651-666
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection. (2015)
  • Author(s): Mbanefo EC, Kumagai T, Kodama Y, Kurosaki T, Furushima-Shimogawara R, Cherif MS, Mizukami S, Kikuchi M, Huy NT, Ohta N, Sasaki H, Hirayama K.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 4 Pages: 24-31
  • DOI: 10.1016/j.parint.2015.01.005
  • Peer Reviewed / Open Access
• [Journal Article] Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines (2014)
  • Author(s): Kato-Hayashi N, Leonardo LR, Arevalo NL, Tagum MNB, Apin J, Agsolid LM, Chua JC, Villacorte EA, Kirinoki M, Kikuchi M, Ohmae H, Haruki K, Chigusa Y
  • Journal Title: Acta Tropica Volume: 141 Pages: 178-183
  • DOI: 10.1016/j.actatropica.2014.05.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant