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New bacterial characteristics based on esp genes

Research Project

Project/Area Number 15K08457
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionUniversity of Tsukuba

Principal Investigator

Morikawa Kazuya  筑波大学, 医学医療系, 教授 (90361328)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords黄色ブドウ球菌 / 不均一性 / esp / 限定的発現遺伝子 / 乾燥耐性 / 集団不均一性
Outline of Final Research Achievements

Survival of only subpopulation of cells (rather than all of the cells) is the ordinary strategy for bacteria to cope with diverse environments. Certain species switches the type of flagellar to escape from host immune system, and others express competence machinery to incorporate new genetic information. In such cases, expression of cryptic genes (genes that ordinary do not express) in subpopulation is important. We have identified a series of such genes, and termed “esp: expression in subpopulation”, but function of many esp genes still elusive. This study aimed to clarify the functions of esp genes, and found that esp17 is responsible for the resistance to dryness.

Academic Significance and Societal Importance of the Research Achievements

本研究で対象とした黄色ブドウ球菌は乾燥に強く、乾燥表面で数ヶ月以上生き残る。ヒト-モノ(例えばドアノブなど)-ヒトという感染経路を成立させる上で乾燥耐性は重要な特質であるが、乾燥耐性の実際のメカニズムは実はよく分かっていない。esp17は高浸透圧での増殖には必要ないものであった。すなわちesp17は未だメカニズムが不明な乾燥耐性に浸透圧耐性以外の仕組みで寄与する因子である可能性がある。その仕組みについて今後明らかにすることでこの重要なヒト病原細菌の新たな側面が明らかになる。

Report

(4 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 7 results) Remarks (3 results)

  • [Int'l Joint Research] Universidad Complutense(Spain)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Horizontal Gene Transmission of cfr gene to MRSA and Enterococcus: role of S. epidermidis as reservoir and alternative pathway for the spread of linezolid resistance.2016

    • Author(s)
      Fabio Cafini, Le Thuy Nguyen Thi, Masato Higashide, Federico Román, José Prieto, and Kazuya Morikawa
    • Journal Title

      J Antimicrob Chemother

      Volume: 71 Issue: 3 Pages: 587-592

    • DOI

      10.1093/jac/dkv391

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cell wall-affecting antibiotics modulate natural transformation in SigH-expressing Staphylococcus aureus.2016

    • Author(s)
      Le Thuy Nguyen Thi, Veronica Medrano Romero, and Kazuya Morikawa
    • Journal Title

      J Antibiot

      Volume: tbd Issue: 6 Pages: 464-466

    • DOI

      10.1038/ja.2015.132

    • NAID

      120007135316

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] 黄色ブドウ球菌が乾燥下で生き抜くメカニズム:限定的発現遺伝子の関与の可能性2017

    • Author(s)
      森川一也、Vishal Samir Gor, 竹村彩、Lisa Maudsdoter
    • Organizer
      第90回日本細菌学会総会 シンポジウム “Sleeping Microbes:眠れる微生物の秘めたる力”
    • Place of Presentation
      仙台国際センター展示棟、宮城県、仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Two esp (expression in minor subpopulation) genes confer dry stress resistance to Staphylococcus aureus.2017

    • Author(s)
      Vishal Samir Gor, Aya Takemura, Kazuya Morikawa.
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター展示棟、宮城県、仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Survival of Staphylococcus aureus under dry conditions: possible involvement of esp genes.2017

    • Author(s)
      Vishal Gor, Aya Takemura, Lisa Maudsdotter, Kazuya Morikaw.
    • Organizer
      Tsukuba Global Science Week
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Survival of Staphylococcus aureus under dry conditions: possible involvement of esp genes.2017

    • Author(s)
      Vishal Gor, Aya Takemura, Lisa Maudsdotter, Kazuya Morikawa.
    • Organizer
      19th International Conference on Bacilli & Gram-Positive Bacteria.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reversible mutations in agr locus in Staphylococcus aureus.2015

    • Author(s)
      Aya J Takemura and Kazuya Morikawa
    • Organizer
      VIth International Conference on Environmental, Industrial, and Applied Microbiology.
    • Place of Presentation
      バルセロナ、スペイン
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Natural competence for DNA transformation in Staphylococcus aureus.2015

    • Author(s)
      Kazuya Morikawa, Nguyen Thi Le Thuy, Aya Takemura, Ryosuke Ohniwa, Tarek Msadek
    • Organizer
      Life and Medical Sciences in South East Asia. TGSW.
    • Place of Presentation
      つくば国際会議場、つくば
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] A part of Agr-deficient ‘stealth’ Staphylococcus aureus can regain its virulence during phagocytosis.2015

    • Author(s)
      Aya Takemura, Kazuya Morikawa
    • Organizer
      The 9th Tsukuba Medical Science Research Meeting.
    • Place of Presentation
      つくば国際会議場、つくば
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Adaptation beyond the stress response: Cell structure dynamics and population heterogeneity in Staphylococcus aureus.2015

    • Author(s)
      Kazuya Morikawa
    • Organizer
      Biomedical Science Forum
    • Place of Presentation
      ジャカルタ、インドネシア
    • Year and Date
      2015-08-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Competence regulators in Gram-positive bacteria.2015

    • Author(s)
      Veronica Medrano Romero, Kazuya Morikawa
    • Organizer
      3rd International Congress on Bacteriology and Infectious Diseases
    • Place of Presentation
      バレンシア、スペイン
    • Year and Date
      2015-08-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 感染生物学(微生物学)研究室

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/microbiology/

    • Related Report
      2016 Research-status Report
  • [Remarks] 筑波大学 医学医療系 微生物学研究室

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/microbiology/index.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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