| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans.Spores are important factors to cause diseases but the mechanism of how to start sporulation has not been identified. One of the sensor proteins to start the sporulation was identified in this research. The mutant strain of this sensor reduced the sporulation and its complemented strain recovered the sporulation rate. Moreover, Clostridium butyricum and C. perfringens were co-cultured in the sporulation condition. These bacteria are normal flora of human intestine and have possibility to communicate to each other. A decrease in sporulation rate of C. perfringens was found, furthermore, transcription of enterotoxin gene was also found to be reduced by the co-culture with C. butyricum. These data indicated that signals from C. butyricum control the gene expression in C. perfringens. This kind of communication of bacteria in the human intestine might be very important to maintain the bacteria balance.
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