• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of peripheral nerve damage by Mycobacterium ulcerans in Buruli ulcer

Research Project

Project/Area Number 15K08466
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionKagoshima University

Principal Investigator

GOTO MASAMICHI  鹿児島大学, 医歯学総合研究科, 客員研究員 (80325779)

Co-Investigator(Kenkyū-buntansha) 鈴木 幸一  帝京大学, 医療技術学部, 教授 (20206478)
圓 純一郎  国際医療福祉大学, 成田保健医療学部, 講師 (30587879)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsMycolactone / 神経傷害機構 / シュワン細胞 / ブルーリ潰瘍 / 抗酸菌感染症 / 末梢神経障害 / アポトーシス / マイコラクトン
Outline of Final Research Achievements

Buruli ulcer is a chronic painless skin disease caused by Mycobacterium ulcerans. The local nerve damage induced by M. ulcerans invasion is similar to the nerve damage evoked by the injection of mycolactone in a Buruli ulcer mouse model. In order to elucidate the mechanism of this nerve damage, we tested and compared the cytotoxic effect of synthetic mycolactone A/B on cultured Schwann cells, fibroblasts and macrophages. Mycolactone induced much higher cell death and apoptosis in Schwann cell line SW10 than in fibroblast line L929. These results suggest that mycolactone is a key substance in the production of nerve damage of Buruli ulcer.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Mycolactone cytotoxicity in Schwann cells could explain nerve damage in Buruli ulcer2017

    • Author(s)
      En Junichiro、Kitamoto Sho、Kawashima Akira、Yonezawa Suguru、Kishi Yoshito、Ishii Norihisa、Goto Masamichi
    • Journal Title

      PLOS Neglected Tropical Diseases

      Volume: 11 Issue: 8 Pages: e0005834-e0005834

    • DOI

      10.1371/journal.pntd.0005834

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mycolactone cytotoxicity in Schwann cells could explain nerve damage in Buruli ulcer2018

    • Author(s)
      En Junichiro、Kitamoto Sho、Kawashima Akira、Yonezawa Suguru、Kishi Yoshito、Ishii Norihisa、Goto Masamichi
    • Organizer
      The U.S.-Japan Cooperative Medical Sciences Program, 52nd Mycobacteria Panel Meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブルーリ潰瘍(M.ulcerans感染症)における神経障害機構の研究~培養細胞におけるMycolactoneのアポトーシス誘導について~2017

    • Author(s)
      圓 純一郎、北本 祥、川島 章、岸 義人、米澤 傑、石井則久、後藤正道
    • Organizer
      第90回日本ハンセン病学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブルーリ潰瘍の起因菌から産生されるMycolactoneの細胞傷害性について2016

    • Author(s)
      後藤正道、圓純一郎
    • Organizer
      第89回日本ハンセン病学会総会・学術大会
    • Place of Presentation
      ホテルヴィレッジ(群馬県吾妻郡草津町)
    • Year and Date
      2016-06-06
    • Related Report
      2016 Research-status Report
  • [Presentation] ブルーリ潰瘍(日本で増加中の難治性皮膚抗酸菌感染症)の病理学的特徴2015

    • Author(s)
      後藤正道
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi