• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of epithelial cells infection by Leptospira interrogans

Research Project

Project/Area Number 15K08467
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Toma Claudia  琉球大学, 医学(系)研究科(研究院), 准教授 (40325832)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsLeptospira / infection / epithelial cells / レプトスピラ / 上皮細胞 / 腎臓 / 感染 / 尿細管上皮細胞 / 細胞死
Outline of Final Research Achievements

Leptospira interrogans is a pathogenic, spirochetal bacterium that is responsible for leptospirosis, an emerging worldwide zoonosis. Leptospires colonize the renal proximal tubules and chronically infect the kidney. Live bacteria are excreted into urine, contaminating the environment. In the present study, we compared the interactions between a virulent, low passage (LP) strain and a cultured-attenuated, high passage (HP) strain with epithelial cells.
Our results suggested that L.interrogans are able to manipulate the host oxidative stress response of epithelial cells. During the long-term colonization, leptospires aggregated and attached to the brush border and membrane vesicles which are involved in the formation of a biofilm-like structure.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Leptospiral 3-hydroxyacyl-CoA dehydrogenase as an early urinary biomarker of leptospirosis2018

    • Author(s)
      Toma C, Koizumi N, Kakita T, Yamaguchi T, Hermawan I, Higa N, Yamashiro T.
    • Journal Title

      Heliyon

      Volume: 4 Issue: 4 Pages: e00616-e00616

    • DOI

      10.1016/j.heliyon.2018.e00616

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 病原細菌の感染と宿主応答の分子論―細菌感染によるインフラマゾーム活性化機構およびレプトスピラの持続感染機構2016

    • Author(s)
      鈴木敏彦、トーマ・クラウディア、高江洲義一、比嘉直美、 仲宗根昇
    • Journal Title

      化学療法の領域

      Volume: 32(2) Pages: 109-114

    • Related Report
      2015 Research-status Report
  • [Presentation] 沖縄本島北部におけるレプトスピラ環境DNAに関する研究2018

    • Author(s)
      水山 克,佐藤 行人, サトウ 恵, 源 利文, トーマ クラウディア
    • Organizer
      第55回レプトスピラ・シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 腎近位尿細管上皮細胞を足場とするレプトスピラの感染戦略2018

    • Author(s)
      トーマ クラウディア
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] レプトスピラ症の迅速診断法の開発2017

    • Author(s)
      トーマ・クラウディア、山口孝治、柿田徹也、小泉信夫、松本亜里奈、仲宗根昇、比嘉直美、村上明一、小林潤、山城哲.
    • Organizer
      第54回レプトスピラ・シンポジウム
    • Place of Presentation
      東北大学青葉山キャンパス、宮城県、仙台市
    • Year and Date
      2017-03-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Interaction of virulent Leptospira interrogans with renal epithelial cell.2017

    • Author(s)
      Claudia Toma, Takayoshi Yamaguchi, Naomi Higa, Arina Matsumoto, Nobuhiko Okura, Noboru Nakasone, Toshihiko Suzuki, Tetsu Yamashiro
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター展示棟、宮城県、仙台市
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Evaluation of a leptospiral enzyme for diagnosis of acute leptospirosis2017

    • Author(s)
      Toma C, Koizumi N, Kakita T, Kyan H, Hermawan I, Higa N, Yamashiro T.
    • Organizer
      10th International Leptospirosis Society Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 病原性レプトスピラの腎臓内における長期感染のメカニズム解析2016

    • Author(s)
      山口孝治、トーマ・クラウディア、比嘉直美、仲宗根昇、山城哲.
    • Organizer
      第69回日本細菌学九州支部会
    • Place of Presentation
      宮崎市民プラザ、宮崎県、宮崎市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 病原性レプトスピラの持続感染機構2016

    • Author(s)
      トーマ・クラウディア、山口孝治、比嘉直美、松本亜里奈、水山克、高江洲義一、仲宗根昇、鈴木敏彦
    • Organizer
      第53 回レプトスピラ・シンポジウム
    • Place of Presentation
      大阪大学 吹田キャンパス 大学院・生命機能研究科 生命システム棟(大阪府吹田市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Remarks] 琉球大学大学院医学研究科細菌学講座webページ

    • URL

      http://bacteriology-u-ryukyu.com

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] レプトスピラ症の診断に用いるレプトスピラ抗原検出用抗体2017

    • Inventor(s)
      トーマ クラウディア
    • Industrial Property Rights Holder
      国立大学法人琉球大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 病原性レプトスピラ抗原検出抗体2017

    • Inventor(s)
      トーマ・クラウディア、山口孝治
    • Industrial Property Rights Holder
      琉球大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-02-07
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi