• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The elucidation of the mechanism of interferon gamma mediated xenophagy against intracellular bacteria

Research Project

Project/Area Number 15K08469
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionOsaka Prefecture University

Principal Investigator

Matsuzawa Takeshi  大阪府立大学, 生命環境科学研究科, 准教授 (80370154)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細胞内寄生菌 / 自然免疫 / 細胞自律的免疫 / マクロファージ / インターフェロン / オートファジー / 細菌感染学 / ゼノファジー
Outline of Final Research Achievements

Professional phagocytes, such as macrophages, have a specialized apparatus for use against invading pathogens. After phagocytosis, pathogens are eliminated by these phagocytes. Intracellular bacteria have survival strategies that interfere with the bactericidal ability of phagocytes. Interferon-γ (IFN-γ) is a potent macrophage activator, and stimulation by this cytokine is critical for cell-autonomous innate immunity against intracellular bacteria. Our group has previously demonstrated that autophagy, a host degradation system, is activated by IFN-γ, and this action contributes to IFN-γ-mediated innate immunity.
Here, we characterized the IFN-γ-mediated selective autophagy against Listeria monocytogenes, and these phenotypes are consistent with LC3-associated phagocytosis (LAP), which has recently emerged as an innate immune response that is related to autophagy. We therefore conclude that LAP is mobilized for IFN-γ-mediated cell-autonomous innate immunity.

Academic Significance and Societal Importance of the Research Achievements

選択的オートファジーは損傷ミトコンドリアの排除等、生体の恒常性維持の観点からだけではなく、細菌を含む病原細菌の感染防御や腫瘍細胞の駆除等、生体防御の観点からも重要である。IFN-γによる選択的オートファジーの活性化メカニズムの一端を解明した本研究は、オートファジーがどのようにして選択性・特異性を上昇させているのかを理解する一助となる。以上のことから本研究により、細菌感染症学領域だけではなく細胞生物学領域における新たな知見が得られたと考えられる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis.2017

    • Author(s)
      Hubber A., Kubori T., Coban C., Matsuzawa T., Ogawa M., Kawabata T., Yoshimori T., and Nagai H.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 44795-44795

    • DOI

      10.1038/srep44795

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] LC3 associated phagocytosis (LAP) はインターフェロンγ誘導性細胞自律的自然免疫に関与する2017

    • Author(s)
      松澤健志
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] イヌインターフェロンγのマクロファージを介した細菌感染防御メカニズムの解明2017

    • Author(s)
      瀬川晶子、熊谷恒平、谷浩行、松澤健志
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] RAW 264.7マクロファージ様細胞を用いた黄色ブドウ球菌感染実験の確立2017

    • Author(s)
      瀬川晶子、松澤健志
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] イヌインターフェロンγのマクロファージを介した感染防御メカニズムの解明2016

    • Author(s)
      瀬川晶子、熊谷恒平、谷浩行、松澤健志
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学 生物資源科学部
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Interferon gamma facilitates autolysosome formation in macrophages2016

    • Author(s)
      Takeshi Matsuzawa
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi