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A comprehensive analysis of the Salmonella type III effectors targets the NF-kappaB signaling pathway

Research Project

Project/Area Number 15K08470
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionKitasato University

Principal Investigator

Haneda Takeshi  北里大学, 薬学部, 講師 (00348591)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsサルモネラ / III型エフェクター / NF-κB / 炎症制御 / サルモネラ属細菌 / NF-κB活性化制御
Outline of Final Research Achievements

We have identified the signal regulated effectors (Sres) which inhibit TNFα-induced NF-κB activation. In this study, we tried to determine the role of Sres in Salmonella pathogensis. SreA, SreB, and SreC are highly homology to one another and have the consensus zinc metalloprotease HEXXH motif. We showed that SreA, SreB, or SreC directly cleaved NF-κB p65, but the protease activity of SreC is lower than that of SreA or SreB. These results suggest that because of their redundant role in cleaving p65, the effect of SreC could be masked by SreA and SreB. Therefore, SreC may not be necessary to block NF-κB signaling in Salmonella infection. Unfortunately, we found no significant differences in the level of CFU in different tissues and intestinal inflammation in mice orally inoculated with S. Typhimurium wild-type or sreAB, or sreABC mutants.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] Salmonella Typhimurium CpxRA two-component system contributes to gut colonization in Salmonella-induced colitis2018

    • Author(s)
      Mayuka Fujimoto, Ryosuke Goto, Takeshi Haneda, Nobuhiko Okada, and Tsuyoshi Miki
    • Journal Title

      Infection and Immunity

      Volume: 00 Issue: 7 Pages: 0000-0000

    • DOI

      10.1128/iai.00280-18

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] サルモネラIII型エフェクターによる炎症抑制機構2016

    • Author(s)
      岡田信彦、羽田健
    • Journal Title

      アレルギーの臨床

      Volume: 36 Pages: 60-65

    • Related Report
      2015 Research-status Report
  • [Presentation] サルモネラT3SS-1非依存的炎症に関与するエフェクターの同定2018

    • Author(s)
      松田茂樹、羽田健、岡田信彦
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Modulation of NF-κB activation by Salmonella type III effectors2017

    • Author(s)
      羽田健、竹村桃、岡田信彦
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター展示棟
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] サルモネラNF-κB制御エフェクターの機能解析2017

    • Author(s)
      羽田健
    • Organizer
      第100回日本細菌学会関東支部総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] サルモネラの炎症制御エフェクターの機能解析2015

    • Author(s)
      羽田健、岡田信彦
    • Organizer
      北里大学微生物アカデミー
    • Place of Presentation
      北里大学相模原キャンパス(相模原、神奈川)
    • Year and Date
      2015-08-06
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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