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Analysis of the species-specific molecular mechanism of C. perfringens-specific lytic enzyme Psm and its application

Research Project

Project/Area Number 15K08482
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionMatsuyama University

Principal Investigator

Tamai Eiji  松山大学, 薬学部, 准教授 (40333512)

Co-Investigator(Kenkyū-buntansha) 吉田 裕美  香川大学, 総合生命科学研究センター, 准教授 (10313305)
成谷 宏文  広島大学, 生物圏科学研究科, 准教授 (30452668)
Co-Investigator(Renkei-kenkyūsha) YOSHIDA Hiromi  香川大学, 総合生命科学研究センター, 准教授 (10313305)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords溶菌酵素 / ウエルシュ菌 / 種特異的 / 細菌細胞壁 / デフィシル菌 / ペプチドグリカン / X線構造解析 / SH3ドメイン / 抗菌薬
Outline of Final Research Achievements

In this study, molecular mechanism of Clostridium perfringens specific lytic enzyme (Psm, Acp, CPE 1138) and its application study were carried out. The species specificity of Psm was found to be in its cell wall binding domain. In addition, enteric coarted preparations of Psm showed some effect on C. perfringens in the intestine. Furthermore, in this study, it was clarified that the catalytic domain of Acp adopts a crescent structure composed of three subdomains, and revealed that the reaction mechanism is Neighboring - group mechanism. In addition, the catalytic domain and cell wall binding domain of CPE 1138 were identified. Furthermore, crystals of the catalytic domain of CPE 1138 were obtained, and data with resolution of 2.0 angstroms was successfully obtained by X-ray structural analysis. Currently, this structural analysis is carried out.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] X-ray structure of Clostridium perfringens sortase B cysteine transpeptidase.2017

    • Author(s)
      Tamai E, Sekiya H, Maki J, Nariya H, Yoshida H, Kamitori S
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 493 Issue: 3 Pages: 1267-1272

    • DOI

      10.1016/j.bbrc.2017.09.144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and biochemical characterization of the Clostridium perfringens autolysin catalytic domain2017

    • Author(s)
      Tamai E, Sekiya H, Goda E, Makihata N, Maki J, Yoshida H, Kamitori S.
    • Journal Title

      FEBS Letter

      Volume: 591 Issue: 1 Pages: 231-239

    • DOI

      10.1002/1873-3468.12515

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 溶菌酵素 Psm に対するタイコ酸の影響2018

    • Author(s)
      関谷 洋志,檜垣 恵二,玉井 栄治,牧 純
    • Organizer
      第91回 日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biocontrol of Clostridium perfringens by using two types of specific endolysins2018

    • Author(s)
      岡田 真歩,玉井 栄治,関谷 洋志,島本 敏,島本 整,成 谷 宏文
    • Organizer
      第91回 日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Expression of glyceraldehyde-3-phosphate dehydrogenase on the Clostridium perfringens cell surface2018

    • Author(s)
      藤本 佳那子,松永 望,玉井 栄治,片山 誠一,櫃本 泰雄
    • Organizer
      第91回 日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウエルシュ菌細胞壁分解酵素Acpの構造と機能解析2017

    • Author(s)
      玉井 栄治、関谷 洋志、巻幡 奈保美、吉田 裕美、神鳥 成弘、牧 純
    • Organizer
      第90回 日本細菌学会 総会
    • Place of Presentation
      宮城県 仙台 仙台国際センター
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 腸内細菌叢構成菌に対する溶菌酵素Psmの影響2017

    • Author(s)
      関谷 洋志,玉井 栄治,牧 純
    • Organizer
      第90回 日本細菌学会 総会
    • Place of Presentation
      宮城県 仙台 仙台国際センター
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Clostridium perfringens由来glyceraldehyde-3-phosphate dehydrogenase (GAPDH)の細菌表層表出とその機能2017

    • Author(s)
      藤本佳那子,松永望,片山誠一,玉井栄治,櫃本泰雄
    • Organizer
      第70回 日本細菌学会中国・四国支部総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウエルシュ菌細胞壁分解酵素の機能解析2017

    • Author(s)
      関谷洋志、玉井栄治、神鳥成弘、牧純
    • Organizer
      第29回 微生物シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 溶菌酵素を用いた腸内環境改善薬の開発2017

    • Author(s)
      玉井 栄治
    • Organizer
      8th Forum of Network Association of Microbiologists in Ehime (NAME)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウエルシュ菌オートライシンAcpの構造と機能解析2016

    • Author(s)
      玉井 栄治,関谷 洋志,牧 純
    • Organizer
      第69回 日本細菌学会 中四国支部会
    • Place of Presentation
      香川県 高松 かがわ国際会議場
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 溶菌酵素 Psm の腸内常在菌に対する作用および変異体解析2016

    • Author(s)
      関谷洋志,玉井栄治,牧純
    • Organizer
      第69回 日本細菌学会 中四国支部会
    • Place of Presentation
      香川県 高松 かがわ国際会議場
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] Clostridium perfringens の T7 発現系の改良2016

    • Author(s)
      澤入 駿哉,鳥谷 采加,野寺 菜美子,玉井 栄治,森山 龍一,宮田 茂
    • Organizer
      日本細菌学会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 溶菌酵素Psmの種特異性に対する細胞壁結合ドメインの影響2016

    • Author(s)
      関谷 洋志,玉井 栄治,成谷 宏文,牧 純
    • Organizer
      日本細菌学会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ウエルシュ菌溶菌酵素オートライシン触媒ドメインのX線結晶解析2015

    • Author(s)
      吉田裕美,神鳥成弘、玉井栄治,関谷洋志、牧純
    • Organizer
      日本生化学会、日本分子生物学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 今日から始める細菌タンパク質の構造機能解析2015

    • Author(s)
      玉井栄治、神鳥成弘
    • Organizer
      日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 溶菌酵素Psmにおける細胞壁結合ドメイン変異体の解析2015

    • Author(s)
      関谷 洋志,玉井 栄治,成谷 宏文,牧 純
    • Organizer
      日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] ウエルシュ菌プロテインキナーゼの解析2015

    • Author(s)
      成谷宏文、玉井栄治、桑原知巳
    • Organizer
      日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Remarks] 松山大学 薬学部 感染症学研究室

    • URL

      http://yakugaku.matsuyama-u.ac.jp/laboratory/kansensyo/saito/Home.html

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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