EprS, an autotransporter serine protease, exerts pleiotropic effects on various pathogenic phenotypes of Pseudomonas aeruginosa
| Project/Area Number |
15K08483
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kurume University |
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Principal Investigator |
KIDA YUTAKA 久留米大学, 医学部, 准教授 (30309752)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 緑膿菌 / オートトランスポーター / プロテアーゼ / 病原性 |
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| Outline of Final Research Achievements |
We previously demonstrated that EprS, an autotransporter serine protease of Pseudomonas aeruginosa, induces host inflammatory responses through protease-activated receptors. However, little is known about the role of EprS as a virulence factor of P. aeruginosa. In this study, to investigate whether EprS participates in the pathogenicity of P. aeruginosa, we characterized various pathogenic phenotypes of the wild-type PAO1 strain and its eprS-disrupted mutant. Our results indicated that eprS plays a role in the growth of P. aeruginosa in the presence of limited nutrients such as a medium containing proteinaceous materials as the sole carbon and nitrogen source. Furthermore, the isogenic eprS mutant showed an attenuation of virulence in leucopenic mice as compared with the wild-type strain. Collectively, these results suggest that EprS exerts pleiotropic effects on various pathogenic phenotypes of P. aeruginosa.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Pseudomonas aeruginosa serA gene is required for bacterial translocation through Caco-2 cell monolayers2017
Author(s)
Yasuda, M., Nagata, S., Yamane, S., Kunikata, C., Kida, Y., Kuwano, K., Suezawa, C., Okuda, J.
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Journal Title
PLoS One
Volume: 12
Issue: 1
Pages: e0169367-e0169367
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Successful infection control for a vancomycin-intermediate Staphylococcus aureus outbreak in an advanced emergency medical service centre2016
Author(s)
Sakai, Y., Qin, L., Miura, M., Masunaga, K., Tanamachi, C., Iwahashi, J., Kida, Y., Takasu, O., Sakamoto, T., Watanabe, H.
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Journal Title
J. Hosp. Infect.
Volume: 92
Issue: 4
Pages: 385-391
DOI
Related Report
Peer Reviewed
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