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Identification of an IS-related Shiga toxin-production system in O157

Research Project

Project/Area Number 15K08484
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KUSUMOTO Masahiro  国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 上級研究員 (40548210)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords腸管出血性大腸菌 / O157 / 志賀毒素 / IS629 / EHEC / IS
Outline of Final Research Achievements

Insertion sequence (IS) elements are the simplest mobile genetic elements, and there are many copies of IS elements in the genome of enterohemorrhagic Escherichia coli (EHEC) O157. In this study, we showed that IS629, the most abundant IS elements in the O157 genome, was responsible for the production of Shiga toxin 2 (Stx2) which is the major virulence factor of EHEC. In O157, the production of Stx2 is known to be enhanced by the SOS response and following the Stx2 phage induction, but IS629 induced Stx2 and the Stx2 phage independently of SOS response. Our data suggest that IS elements promote not only bacterial genome diversification with their mobility but also toxin production in O157.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan.2016

    • Author(s)
      Kusumoto M, Hikoda Y, Fujii Y, Murata M, Miyoshi H, Ogura Y, Gotoh Y, Iwata T, Hayashi T, Akiba M.
    • Journal Title

      J Clin Microbiol.

      Volume: 54 Issue: 4 Pages: 1074-81

    • DOI

      10.1128/jcm.03141-15

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Colistin-resistant mcr-1-positive pathogenic Escherichia coli in swine, Japan, 2007-20142016

    • Author(s)
      Kusumoto M, Ogura Y, Gotoh Y, Iwata T, Hayashi T, Akiba M
    • Journal Title

      Emerging Infectious Diseases

      Volume: 22 Issue: 7 Pages: 1315-1317

    • DOI

      10.3201/eid2207.160234

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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