| Project/Area Number |
15K08486
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
IYODA SUNAO 国立感染症研究所, 細菌第一部, 室長 (70300928)
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| Project Period (FY) |
2015-10-21 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腸管出血性大腸菌 / 志賀毒素 / LEE非保有型 / 腸管凝集接着性大腸菌 / 接着因子 |
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| Outline of Final Research Achievements |
We have isolated several LEE-negative enterohemorrhagic E. coli (EHEC) from patients with severe symptoms, and found that one of them was a "hybrid strain" that are belonging to both EHEC and enteroaggregative E. coli (EAggEC) categories. Using whole genome sequence analysis, we found that this strain possesses a Shiga-toxin 2 converting phage which has highly similar DNA sequences (only few SNPs) to those found in a similar EAggEHEC hybrid strain serotyped O104:H4, cause of massive outbreak resulting several hundreds of HUS cases and more than 50 deaths occurred in Europe, 2011.
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| Academic Significance and Societal Importance of the Research Achievements |
重症例由来の非定型な（LEE非保有型）EHECの解析から、EHECとは別の下痢原性大腸菌カテゴリーである腸管凝集接着性大腸菌（EAggEC）とEHECのハイブリッド株が複数分離されることが判明した。これらのハイブリッド株の一つが保有するStx2ファージの完全長配列を次世代シークエンサーで解析したところ、2011年に欧州各国で大発生したEHECとEAggECのハイブリッド株O104:H4が保有するStx2ファージの配列と数SNPsの違いしかないほぼ同一のファージであることが判明した。本研究の成果は、完全長配列解析によってStxファージの動態を解析し得る可能性を示した点において意義深い。
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