• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Control of Chronic Liver Diseases due to Hepatitis C Virus by Long Non-coding RNAs

Research Project

Project/Area Number 15K08492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionKanazawa University

Principal Investigator

Shimakami Tetsuro  金沢大学, 附属病院, 特任教授 (50436820)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
KeywordsC型肝炎ウイルス / 長鎖ノンコーディングRNA / C型慢性肝炎 / ノンコーディングRNA
Outline of Final Research Achievements

We investigated the role of long non-coding RNAs in HCV infection. The analysis on RNA of HCV-infected cultured hepatocytes by a next generation sequencer revealed that the RNA level of one of long non-coding RNAs, HULC, increased. The increase of HULC RNA level was observed not only in HCV-infected cultured hepatocytes, but also in an HCV-infected chimpanzee and a HCV-infected mouse. The knockdown of HCV by siRNA suppressed HCV replication as well as infectious virus production in HCV-infected cultured hepatocytes. This effect seemed to occur through the suppression of a translation step among HCV life cycles. Several recent reports show that HULC has the ability to promote hepatocarcinogenesis, therefore, the result of this study suggests a mechanism of hepatocarcinogesis due to HCV infection through HULC.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (7 results) (of which Int'l Joint Research: 5 results) Book (1 results)

  • [Journal Article] Peretinoin, an Acyclic Retinoid, Inhibits Hepatitis B Virus Replication by Suppressing Sphingosine Metabolic Pathway In Vitro.2018

    • Author(s)
      1.Murai K, Shirasaki T, Honda M, Shimizu R, Shimakami T, Nakasho S, Shirasaki N, Okada H, Sakai Y, Yamashita T, Kaneko S.
    • Journal Title

      International Journal of Molecular Sciences.

      Volume: 19(2) Issue: 2 Pages: 108-108

    • DOI

      10.3390/ijms19020108

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Unexpected Replication Boost by Simeprevir for Simeprevir-resistant Variants in Genotype 1a Hepatitis C Virus2018

    • Author(s)
      Murai K, Shimakami T, Welsch C, Shirasaki T, Liu F, Kitabayashi J, Tanaka S, Funaki M, Omura H, Nishikawa T, Suminyadorj A, Honda M, Kaneko S.
    • Journal Title

      Antimicrob Agents Chemother

      Volume: 印刷中 Issue: 7

    • DOI

      10.1128/aac.02601-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Peretinoin, an acyclic retinoid, inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo.2017

    • Author(s)
      Funaki M, Kitabayashi J, Shimakami T, Nagata N, Sakai Y, Takegoshi K, Okada H, Murai K, Shirasaki T, Oyama T, Yamashita T, Ota T, Takuwa Y, Honda M, Kaneko S.
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 16978-16978

    • DOI

      10.1038/s41598-017-17285-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reactivation of hepatitis B virus from an isolated anti-HBc positive patient after eradication of hepatitis C virus with direct-acting antiviral agents2017

    • Author(s)
      Suda Tsuyoshi、Shimakami Tetsuro、Shirasaki Takayoshi、Yamashita Tatsuya、Mizukoshi Eishiro、Honda Masao、Kaneko Shuichi
    • Journal Title

      J Hepatol

      Volume: 67 Issue: 5 Pages: 1108-1111

    • DOI

      10.1016/j.jhep.2017.07.014

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hepatitis B virus X protein induces hepatic stem cell-like features in hepatocellular carcinoma by activating KDM5B.2017

    • Author(s)
      Wang X, Oishi N, Shimakami T, Yamashita T, Honda M, Murakami S, Kaneko S.
    • Journal Title

      World J Gastroenterol

      Volume: 23 Issue: 18 Pages: 3252-3261

    • DOI

      10.3748/wjg.v23.i18.3252

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3' variants.2017

    • Author(s)
      Yamane D, Selitsky SR, Shimakami T, Li Y, Zhou M, Honda M, Sethupathy P, Lemon SM.
    • Journal Title

      Nucleic Acids Res.

      Volume: - Pages: 4743-4755

    • DOI

      10.1093/nar/gkw1332

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient Suppression of Hepatitis C Virus Replication by Combination Treatment with miR-122 Antagonism and Direct-acting Antivirals in Cell Culture Systems.2016

    • Author(s)
      Liu F, Shimakami T, Murai K, Shirasaki T, Funaki M, Honda M, Murakami S, Yi M, Tang H, Kaneko S.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 30939-30939

    • DOI

      10.1038/srep30939

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 当院における肝移植後C型肝炎ウイルス感染に対するオムビタスビル水和物・パリタプレビル水和物・リトナビル配合錠の使用経験2017

    • Author(s)
      島上哲朗,砂子阪肇,金子周一
    • Organizer
      第103回日本消化器病学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アスナプレビル・ダクラタスビル療法の治療効果と非著効例に対するレジパスビル・ソフォスブビルの治療効果に関する検討2017

    • Author(s)
      島上哲朗,本多政夫,金子周一
    • Organizer
      第53回日本肝臓学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Unexpected Enhancement of Replication Capacity by Simeprevir for Simeprevir-resistant Variants in Genotype 1a Hepatitis C Virus2017

    • Author(s)
      Tetsuro Shimakami, Kazuhisa Murai, Christoph Welsch, Takayosi Shirasaki, Masao Honda, and Shuichi Kaneko
    • Organizer
      24th International Symposium on Hepatitis C Virus and Related Viruses
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unexpected Enhancement of Replication Capacity by Simeprevir for Simeprevir-resistant Variants in Genotype 1a Hepatitis C Virus2017

    • Author(s)
      Tetsuro Shimakami, Kazuhisa Murai, Christoph Welsch, Takayosi Shirasaki, Masao Honda, and Shuichi Kaneko
    • Organizer
      The Liver Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effective Prevention of Direct-acting Antiviral-resistant Hepatitis C Virus by Combination with Anti-miR-122 Therapy in Cell Culture2016

    • Author(s)
      Tetsuro Shimakami, Fanwei Liu, Kazuhisa Murai, Takayoshi Shirasaki, Masao Honda, Shuichi Kaneko
    • Organizer
      AASLD 2016
    • Place of Presentation
      ボストン
    • Year and Date
      2016-11-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of Hepatitis C Virus Infection by Long Non-Coding RNA2015

    • Author(s)
      Shimakami T, Honda M, Shirasaki T, Liu F, Funaki M, Murai K, Kaneko S
    • Organizer
      The 66th Annual Meeting of the American Association for the Study of Liver Diseases
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of Hepatitis C Virus Infection by Long Non-Coding RNA2015

    • Author(s)
      Shimakami T, Honda M, Shirasaki T, Liu F, Funaki M, Murai K, Ooishi N, Kaneko S
    • Organizer
      22nd International Symposium on Hepatitis C Viruses and Related Viruses
    • Place of Presentation
      ストラスブール(フランス)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 消化器の臨床2016

    • Author(s)
      島上哲朗、金子周一
    • Total Pages
      7
    • Publisher
      ヴァンメディカル
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi