| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
The sequence of Borna disease virus (BDV) is reverse transcribed and integrated into the host genome. It is thought that this reaction is regulated by the interaction between BDV and LINE (long interspersed nuclear element), a host retrotransposon. However, the physiological significance of this phenomenon and the physiological function of LINE itself were still unknown. In this study, we elucidated the interaction between virus and LINE and the physiological significance of virus-derived sequences in the genome generated by the virus-retrotransposon interaction. We found that BDV infection indeed affects LINE activity, that BDV-related sequences in the mouse genome (mmEBLs) produce mmEBL-derived piRNAs, and that the mmEBL-derived piRNAs suppress mRNA with BDV-related sequences. From these results, we have proposed that LINE promotes the uptake of viral sequences into the host genome and imparts virus resistance to the host.
|
