Interactions between retrotransposon and RNA viruses
| Project/Area Number |
15K08496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University (2016-2017)
Kyoto University (2015) |
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Principal Investigator |
Honda Tomoyuki 大阪大学, 医学系研究科, 准教授 (80402676)
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| Research Collaborator |
SOFUKU Kozue
NAKAYAMA Ryota
NISHIKAWA Yuki
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | レトロトランスポゾン / RNAウイルス / ボルナ病ウイルス / 感染防御機構 / 内在性ウイルス配列 / piRNA / B型肝炎ウイルス |
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| Outline of Final Research Achievements |
The sequence of Borna disease virus (BDV) is reverse transcribed and integrated into the host genome. It is thought that this reaction is regulated by the interaction between BDV and LINE (long interspersed nuclear element), a host retrotransposon. However, the physiological significance of this phenomenon and the physiological function of LINE itself were still unknown. In this study, we elucidated the interaction between virus and LINE and the physiological significance of virus-derived sequences in the genome generated by the virus-retrotransposon interaction. We found that BDV infection indeed affects LINE activity, that BDV-related sequences in the mouse genome (mmEBLs) produce mmEBL-derived piRNAs, and that the mmEBL-derived piRNAs suppress mRNA with BDV-related sequences. From these results, we have proposed that LINE promotes the uptake of viral sequences into the host genome and imparts virus resistance to the host.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.2016
Author(s)
Horie M, Kobayashi Y, Honda T, Fujino K, Akasaka T, Kohl C, Wibbelt G, Muhldorfer K, Kurth A, Muller MA, Corman VM, Gillich N, Suzuki Y, Schwemmle M, Tomonaga K.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 25873-25873
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] piRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals.2015
Author(s)
Parrish NF, Fujino K, Shiromoto Y, Iwasaki YW, Ha H, Xing J, Makino A, Kuramochi-Miyagawa S, Nakano T, Siomi H, Honda T, Tomonaga K.
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Journal Title
RNA.
Volume: 21
Issue: 10
Pages: 1691-703
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Influenza A Virus-Induced Expression of a GalNAc Transferase, GALNT3, via MicroRNAs Is Required for Enhanced Viral Replication.2015
Author(s)
Nakamura S, Horie M, Daidoji T, Honda T, Yasugi M, Kuno A, Komori T, Okuzaki D, Narimatsu H, Nakaya T, Tomonaga K.
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Journal Title
J Virol.
Volume: 90
Issue: 4
Pages: 1788-801
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Induction of stress responses in mouse skeletal muscles expressing measles virus nucleocapsid protein (MV-N)2016
Author(s)
Kwon H, Watanabe Y, Nakanishi T, Honda T, Sato H, Sugai A, Uchida S, Nakae S, Ogonuki N, Ogura A, Yoneda M, Kai C.
Organizer
第64回日本ウイルス学会学術集会
Place of Presentation
横浜
Related Report
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[Presentation] 新規鳥ボルナウイルス(PaBV-5)の配列解析2015
Author(s)
Ryo Komorizono, Masayuki Horie, Tomoyuki Honda, Akiko Makino, Keizo Tomonaga
Organizer
第63回日本ウイルス学会学術集会
Place of Presentation
福岡
Year and Date
2015-11-22
Related Report
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