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Characterization of avian influenza virus variants isolated from infected patients

Research Project

Project/Area Number 15K08497
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Watanabe Yohei  京都府立医科大学, 医学(系)研究科(研究院), 講師 (50452462)

Co-Investigator(Kenkyū-buntansha) 鈴木 康夫  中部大学, 生命健康科学部, 客員教授 (00046278)
大道寺 智  京都府立医科大学, 医学(系)研究科(研究院), 講師 (80432433)
Co-Investigator(Renkei-kenkyūsha) ARAI Yasuha  京都府立医科大学, 医学研究科, 博士研究員 (80793182)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsウイルス学 / 人獣共通感染症学 / 感染病態学 / インフルエンザウイルス
Outline of Final Research Achievements

Our database search of avian H5N1 influenza viruses identified 59 HA mutations that had been selected in infected patients. Using reverse genetics, we found that H5N1 variants with some of these HA mutations had increased human-type receptor specificity and increased HA membrane fusion activity, both of which are advantageous for viral replication in human airway epithelia. Furthermore, HA mutants selected during viral replication in patients were likely to have less HA stability, apparently as compensatory mechanism. Our findings showed that H5N1 viruses could rapidly adapt to growth in the human airway microenvironment by altering HA properties in infected patients and provided new insights into the human-adaptive mechanisms of avian influenza viruses.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (41 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (24 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] Alexandria University/Damanhour University(エジプト)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Thammasat University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Alexandria University/Damanhour University(エジプト)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Alexandria University/Damanhour University(エジプト)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Characterization of H5N1 influenza virus quasispecies with adaptive hemagglutinin mutations from single-virus infections of human airway cells2018

    • Author(s)
      1.Watanabe Y**, Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Kajikawa J, Hiramatsu H, Sriwilaijaroen N, Ono T, Takagi T, Takahashi K, Shioda T, Matsumoto K, Suzuki Y, Nakaya T
    • Journal Title

      J Virol.

      Volume: 印刷中 Issue: 11

    • DOI

      10.1128/jvi.02004-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Unique Infectious Strategy of H5N1 Avian Influenza Virus Is Governed by the Acid-Destabilized Property of Hemagglutinin.2017

    • Author(s)
      Daidoji T**, Watanabe Y, Arai Y, Kajikawa J, Hirose R, Nakaya T
    • Journal Title

      Viral Immunol.

      Volume: 30 Issue: 6 Pages: 398-407

    • DOI

      10.1089/vim.2017.0020

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanism of Human Influenza Virus RNA Persistence and Virion Survival in Feces: Mucus Protects Virions From Acid and Digestive Juices.2017

    • Author(s)
      Hirose R, Nakaya T**, Naito Y, Daidoji T, Watanabe Y, Yasuda H, Konishi H, Itoh Y.
    • Journal Title

      J Infect Dis.

      Volume: 216 Issue: 1 Pages: 105-109

    • DOI

      10.1093/infdis/jix224

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of polymerase gene mutations that affect viral replication in H5N1 influenza viruses isolated from pigeons.2017

    • Author(s)
      Elgendy EM, Arai Y, Kawashita N, Daidoji T, Takagi T, Ibrahim MS, Nakaya T, Watanabe Y
    • Journal Title

      J Gen Virol.

      Volume: 98 Issue: 1 Pages: 6-17

    • DOI

      10.1099/jgv.0.000674

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Glycan-functionalized graphene-FETs toward selective detection of human-infectious avian influenza virus2017

    • Author(s)
      Takao Ono
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 56 Issue: 3 Pages: 030302-030302

    • DOI

      10.7567/jjap.56.030302

    • NAID

      210000147449

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses2016

    • Author(s)
      Arai Y, Kawashita N, Daidoji T, Ibrahim MS, El-Gendy EM, Takagi T, Takahashi K, Suzuki Y, Ikuta K, Nakaya T, Shioda T, Watanabe Y
    • Journal Title

      PLoS Pathog.

      Volume: 12 Issue: 4 Pages: e1005583-e1005583

    • DOI

      10.1371/journal.ppat.1005583

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic characterization of highly pathogenic avian influenza H5N1 viruses isolated from naturally infected pigeons in Egypt2016

    • Author(s)
      Elgendy EM, Watanabe Y, Daidoji T, Arai Y, Ikuta K, Ibrahim MS, Nakaya T
    • Journal Title

      Virus Genes

      Volume: 52 Issue: 6 Pages: 867-871

    • DOI

      10.1007/s11262-016-1369-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Long-term detection of seasonal influenza RNA in faeces and intestine.2016

    • Author(s)
      Hirose R, Daidoji T, Naito Y, Watanabe Y, Arai Y, Oda T, Konishi H, Yamawaki M, Itoh Y, Nakaya T.
    • Journal Title

      Clin Microbiol Infect

      Volume: 22 Issue: 9 Pages: 813-813

    • DOI

      10.1016/j.cmi.2016.06.015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of H5N1 Influenza Virus Variants with Hemagglutinin Mutations Isolated from Patients.2015

    • Author(s)
      1.Watanabe Y, Arai Y, Daidoji T, Kawashita N, Ibrahim MS, El-Gendy E, Hiramatsu H, Kubota-Kokestu R, Takagi T, Murata T, Takahashi K, Okuno Y, Nakaya T, Suzuki Y, Ikuta K.
    • Journal Title

      mBio

      Volume: 6 Issue: 2

    • DOI

      10.1128/mbio.00081-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] H5N1鳥インフルエンザウイルスHAの宿主適応機構2015

    • Author(s)
      渡邊洋平、大道寺智、中屋隆明
    • Journal Title

      ウイルス

      Volume: 65 Pages: 187-198

    • NAID

      130005431375

    • Related Report
      2015 Research-status Report
  • [Presentation] Characterization of H5N1 influenza virus variants with adaptive mutations isolated from patients2017

    • Author(s)
      Watanabe Y
    • Organizer
      The Sixth China-Japan Bilateral Symposium on All Influenza Viruses
    • Place of Presentation
      Beijing, China
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 季節性インフルエンザウイルスの腸管感染とそのメカニズムについて2017

    • Author(s)
      中屋隆明
    • Organizer
      6th Negative Strand Virus-Japan Symposium
    • Place of Presentation
      沖縄
    • Year and Date
      2017-01-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Changing nature of H5N1 avian influenza virus in Egypt.2017

    • Author(s)
      Watanabe Y.
    • Organizer
      2nd Kyoto International Symposium on Virus-Host Coevolution.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Adaptive change of H5N1 avian influenza virus circulating in Egypt.2017

    • Author(s)
      Watanabe Y.
    • Organizer
      Interim workshop for project on infectious diseases in Vietnam and development of application in diagnosis, prevention and treatment.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of H5N1 influenza virus with HA mutations diversified in human airway epithelial cells as minor variants.2017

    • Author(s)
      Watanabe Y.
    • Organizer
      JSPS Core-to-Core Program 2nd Scientific Seminar
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of H5N1 influenza virus with HA mutations diversified in human airway epithelial cells as minor variants.2017

    • Author(s)
      Watanabe Y, Arai Y, Kawashita N, Daidoji T, Elgendy EM, Ibrahim MS, Kajikawa J, Hiramatsu H, Takagi T, Takahashi K, Shioda T, Suzuki Y, Nakaya T.
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel polymerase gene mutations for human adaptation in clinical isolates of Avian H5N1 influenza virus in Asia.2017

    • Author(s)
      Arai Y, Kawashita N, Hotta K, Hoang MPV, Nguyen KHL, Nguyen TC, Vuong CD, Le MQ, Soda K, Kajikawa J, Daidoji T, Nakaya T, Takagi T, Ito T, Hasebe H, Shioda T, Watanabe Y.
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evaluation of avian influenza virus infection in differentiated airway epithelial cells.2017

    • Author(s)
      Daidoji T, Noguchi M, Kajikawa J, Watanabe Y, Arai Y, Hirose R, Morimoto M, Nakaya T.
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Mechanism of seasonal influenza virus persistence in human digestive tract: Mucus protects virions from acid and digestive juices.2017

    • Author(s)
      Nakaya T, Hirose R, Daidoji T, Naito Y, Watanabe Y, Arai Y, Kawada K, Kajikawa J, Yamawaki M, Itoh Y.
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Establishment of immortalized cell clones from human tracheal epithelial cells and their different susceptibility to influenza viruses.2017

    • Author(s)
      Kajikawa J, Daidoji T, Watanabe Y, Arai Y, Hirose R, Nakaya T.
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Characterization of H5N1 influenza virus variants with HA mutations selected during the viral replication in human airway epithelial cells2016

    • Author(s)
      Watanabe Y
    • Organizer
      Egypt-Japan First Forum. The Year of Education and Research Collaboration
    • Place of Presentation
      Damanhour, Egypt
    • Year and Date
      2016-12-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The Kaizen way to self-improvement2016

    • Author(s)
      Watanabe Y
    • Organizer
      Egypt-Japan First Forum. The Year of Education and Research Collaboration
    • Place of Presentation
      Damanhour, Egypt
    • Year and Date
      2016-12-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of H5N1 influenza virus variants with HA mutations selected during the viral replication in human airway epithelial cells2016

    • Author(s)
      Watanabe Y, Arai Y, Daidoji T, Elgendy EM, Ibrahim MS, Takahashi K, Shioda T, Ikuta K, Suzuki Y, Nakaya T
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Hemagglutinin acid stability and viral infection in H7 influenza virus2016

    • Author(s)
      Daidoji T, Elgendy EM, Watanabe Y, Arai Y, Ibrahim MS, Ikuta K, Nakaya T
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Novel polymerase gene mutations for human adaptation in clinical isolates of avian H5N1 influenza viruses2016

    • Author(s)
      Arai Y, Kawashita N, Daidoji T, Ibrahim MS, Takayashi K, Suzuki Y, Nakaya T, Shioda T, Ikuta K, Watanabe Y
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of unique mutations that influence the polymerase activity of H5N1 avian influenza virus isolated from pigeons2016

    • Author(s)
      Elgendy EM, Arai Y, Ibrahim MS, Daidoji T, Ikuta K, Nakaya T, Watanabe Y
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Novel Polymerase Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses2016

    • Author(s)
      Watanabe Y
    • Organizer
      The 11th Japan-China International Conference of Virology
    • Place of Presentation
      香川
    • Year and Date
      2016-07-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 鳥インフルエンザ-H5N1/H7N9を中心に-2016

    • Author(s)
      中屋隆明
    • Organizer
      第31回日本環境感染症学会総会
    • Place of Presentation
      京都
    • Year and Date
      2016-02-19
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] H7鳥インフルエンザにおけるHA蛋白質のpH感受性について2016

    • Author(s)
      大道寺 智
    • Organizer
      5th Negative Strand Virus-Japan Symposium
    • Place of Presentation
      沖縄
    • Year and Date
      2016-01-25
    • Related Report
      2015 Research-status Report
  • [Presentation] Genetic diversification of H5N1 avian influenza virus variants during replication in human airway epithelial cells2015

    • Author(s)
      Yohei Watanabe, Yasuha Arai, Tomo Daidoji, Madiha S Ibrahim, Emad Elgendy, Kazuo Takahashi, Yasuo Suzuki, Tatsuo Shioda, Kazuyoshi Ikuta, Takaaki Nakaya
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Dynamics of influenza A virus infection in mucin-type glycosyltransfererase Galnt3-knockout mice2015

    • Author(s)
      Shoko Nakamura, Tomo Daidoji, Tomoyuki Honda, Takaaki Nakaya, Toshihisa Komori, Keizo Tomonaga
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Hemagglutinin acid stability in H7 influenza virus infection to host cells2015

    • Author(s)
      Tomo Daidoji, Emad Elgendy, Yohei Watanabe, Kazuyoshi Ikuta, Takaaki Nakaya
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Identification of human adaptive mutations in the polymerase complex of H5N1 avian influenza virus in Egypt2015

    • Author(s)
      Yasuha Arai, Tomo Didoji, Madiha S. Ibrahim, Kazuo Takahashi, Yasuo Suzuki, Takaaki Nakaya, Tatsuo Shioda, Kazuyoshi Ikuta, Yohei Watanabe
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Genetic characterization of H5N1 avian influenza virus from pigeon in Egypt 20092015

    • Author(s)
      Emad Elgendy, Madiha S. Ibrahim, Arai Yasuha, Tomo Daidoji, Yohei Watanabe, Takaaki Nakaya
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Book] 鳥インフルエンザウイルスの糖鎖を介したヒト適応機構とパンデミック対策2016

    • Author(s)
      渡邊洋平
    • Publisher
      Glycoforum
    • Related Report
      2015 Research-status Report
  • [Remarks] 京都府立医科大学・医学研究科・感染病態学

    • URL

      http://www.f.kpu-m.ac.jp/k/did/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Remarks] 京都府立医科大学・医学研究科・感染病態学教室HP

    • URL

      http://www.f.kpu-m.ac.jp/k/did/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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