| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In order to elucidate the selective assembly mechanism of influenza virus segmented RNA genome-protein complex (vRNP), we attempted to establish an artificial vRNP reconstitution system and to demonstrate the interaction between specific segments by coprecipitation experiments. We also tried to identify the segment assembly/packaging signal sequences present at the termini of each segment by competitive packaging experiments using model virus genome. Since it was suggested as the results that vRNP must be tethered to the membrane surface and laterally diffused in order to selectively assemble, we considered that it is difficult to reproduce such a situation in vitro. It was also suggested that the sequences contributing to the selective assembly or controlling the genome packaging coexist at the termini of viral RNA segment.
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