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Elucidation of the selective assembly mechanism of influenza virus segmented genome using reconstituted viral ribonucleoprotein complexes

Research Project

Project/Area Number 15K08503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionKitasato University

Principal Investigator

MOMOSE Fumitaka  北里大学, 感染制御科学府, 講師 (90332204)

Research Collaborator KAWAHARA MADOKA  北里大学, 北里生命科学研究所, 臨時職員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsインフルエンザウイルス / ポリシストロニック発現 / 自己開裂2A配列 / RNP複合体 / パッケージングシグナル / 競合パッケージング / 選択的分節集合 / モデルウイルスゲノム / RNA依存性RNAポリメラーゼ
Outline of Final Research Achievements

In order to elucidate the selective assembly mechanism of influenza virus segmented RNA genome-protein complex (vRNP), we attempted to establish an artificial vRNP reconstitution system and to demonstrate the interaction between specific segments by coprecipitation experiments. We also tried to identify the segment assembly/packaging signal sequences present at the termini of each segment by competitive packaging experiments using model virus genome. Since it was suggested as the results that vRNP must be tethered to the membrane surface and laterally diffused in order to selectively assemble, we considered that it is difficult to reproduce such a situation in vitro. It was also suggested that the sequences contributing to the selective assembly or controlling the genome packaging coexist at the termini of viral RNA segment.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Polycistronic Expression of the Influenza A Virus RNA-Dependent RNA Polymerase by Using the Thosea asigna Virus 2A-Like Self-Processing Sequence.2016

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 288-288

    • DOI

      10.3389/fmicb.2016.00288

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 蛍光in situ hybridizationによるインフルエンザウイルスゲノム分節末端領域のアクセシビリティ評価2018

    • Author(s)
      百瀬 文隆、森川 裕子
    • Organizer
      7th Negative Strand Virus-Japan
    • Related Report
      2017 Annual Research Report
  • [Presentation] Accessibility assessment of the terminal region of influenza A virus genome segments by fluorescence in situ hybridization.2017

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      第65回 日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Accessibility assessment of the terminal region of influenza A virus genome segments by fluorescence in situ hybridization.2017

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 再構成vRNP複合体を用いたA型インフルエンザウイルスゲノム分節間相互作用の評価2017

    • Author(s)
      百瀬 文隆、森川 裕子
    • Organizer
      6th Negative Strand Virus-Japan
    • Place of Presentation
      沖縄ラグナガーデンホテル(沖縄県・宜野湾市)
    • Related Report
      2016 Research-status Report
  • [Presentation] インフルエンザウイルスRdRp恒常発現細胞樹立の試み2016

    • Author(s)
      百瀬 文隆
    • Organizer
      5th Negative Strand Virus-Japan
    • Place of Presentation
      ホテルモントレ沖縄(沖縄県・恩納村)
    • Year and Date
      2016-01-25
    • Related Report
      2015 Research-status Report
  • [Presentation] Assessment of the potential interaction between the genome segments of influenza A virus by using reconstituted vRNP complexes2016

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      第64回 日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 再構成vRNP複合体を用いたA型インフルエンザウイルスゲノム分節間相互作用の評価2016

    • Author(s)
      百瀬 文隆、森川 裕子
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Establishment of a stable cell line expressing influenza A virus RNA-dependent RNA polymerase.2015

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      第63回 日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Polycistronic expression of the influenza a virus RNA-dependent RNA polymerase by using a 2A self-processing sequence.2015

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      第2回感染コンピテンシー若手研究会
    • Place of Presentation
      マホロバ・マインズ三浦(神奈川県・三浦市)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Polycistronic expression of the influenza a virus RNA-dependent RNA polymerase by using a 2A self-processing sequence.2015

    • Author(s)
      Fumitaka Momose, Yuko Morikawa
    • Organizer
      16th International Conference on Negative Strand Viruses
    • Place of Presentation
      Siena, Italy
    • Year and Date
      2015-06-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 1.インフルエンザウイルスゲノムRNA-タンパク質複合体の検出・輸送・再構成2015

    • Author(s)
      百瀬 文隆
    • Organizer
      第29回インフルエンザ研究者交流の会シンポジウム
    • Place of Presentation
      東京大学医科学研究所(東京都・港区)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Research-status Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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