| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Kaposi’s sarcoma associated herpesvirus expresses immune evasion molecule family MIR, which is membrane-bound E3 ubiquitin ligases to downregulate several immune proteins on host cell surface. Our previous study revealed that MIR recognizes substrates through transmembrane and extracellular region. In this study, to reveal molecular basis of substrate recognition by MIR, recombinant MIR protein containing two transmembrane regions and extracellular region was prepared and reconstructed in nanodisc, which is artificial lipid bilayer. MIR-nanodisc was subjected to solution NMR analysis; however, HSQC signal was too low to determine structure of MIR. In order to improve molecular stability of MIR, we performed mutational analysis of MIR and revealed that cytoplasmic tail of MIR is essential to stabilize MIR structure.
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