| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Reverse genetics is a powerful tool for studying virus replication and pathogenicity and also for vaccine development. We succeeded to develop an entirely plasmid-based reverse genetics system based on the transfection of 14 plasmids (11 rotavirus cDNAs in combination with three helper expression plasmids derived from other viruses). We recently developed an optimized reverse genetics system based on only rotavirus cDNAs (11-plasmid system), in which the concentration of cDNA plasmids containing NSP2 and NSP5 genes is 3- or 5-fold increased in relation to that of the other plasmids. In addition to infectious recombinant rotaviruses expressing fluorescent proteins (EGFP and mCherry), authentic human rotavirus (strain KU (G1P[8])) was also generated with the 11-plasmid system.
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