| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Rubella virus (RUBV) is highly infectious to culture cell lines derived from human trophoblasts but not many other human cell lines. In this study, we have found that almost of adhesion cell lines which we have tested are permissive to the RUBV-entry, while almost of suspension immune cell lines are not. Furthermore, we have identified that sphingomyelin (SM) and cholesterol (Chol) on the cell surface are participate in attachment of RUBV to the cell surface. The envelope protein E1 of RUBV attaches to SM/Chol-rich membrane and induces membrane fusion between cell and RUBV via its fusion loop in the presence of a Ca ion. RUBV also attaches a host proteinous molecule(s), which is not yet identified.This attachment is closely related to cell-permissibility of the RUBV-entry.
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