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Study on a mechanism underlying a transplacental transmission of rubella virus

Research Project

Project/Area Number 15K08508
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Mori Yoshio  国立感染症研究所, ウイルス第三部, 室長 (40379095)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords風疹ウイルス / ウイルス受容体 / スフィンゴミエリン / コレステロール / レセプター
Outline of Final Research Achievements

Rubella virus (RUBV) is highly infectious to culture cell lines derived from human trophoblasts but not many other human cell lines. In this study, we have found that almost of adhesion cell lines which we have tested are permissive to the RUBV-entry, while almost of suspension immune cell lines are not. Furthermore, we have identified that sphingomyelin (SM) and cholesterol (Chol) on the cell surface are participate in attachment of RUBV to the cell surface. The envelope protein E1 of RUBV attaches to SM/Chol-rich membrane and induces membrane fusion between cell and RUBV via its fusion loop in the presence of a Ca ion. RUBV also attaches a host proteinous molecule(s), which is not yet identified.This attachment is closely related to cell-permissibility of the RUBV-entry.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results)

  • [Journal Article] Analysis of VSV pseudotype virus infection mediated by rubella virus envelope proteins2017

    • Author(s)
      Sakata Masafumi、Tani Hideki、Anraku Masaki、Kataoka Michiyo、Nagata Noriyo、Seki Fumio、Tahara Maino、Otsuki Noriyuki、Okamoto Kiyoko、Takeda Makoto、Mori Yoshio
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-14

    • DOI

      10.1038/s41598-017-10865-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Both Sphingomyelin and Cholesterol in the Host Cell Membrane Are Essential for Rubella Virus Entry2017

    • Author(s)
      Otsuki Noriyuki、Sakata Masafumi、Saito Kyoko、Okamoto Kiyoko、Mori Yoshio、Hanada Kentaro、Takeda Makoto
    • Journal Title

      Journal of Virology

      Volume: 92 Issue: 1

    • DOI

      10.1128/jvi.01130-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Epidemiology of Rubella Virus Strains Detected Around the Time of the 2012?2013 Epidemic in Japan2017

    • Author(s)
      Mori Yoshio、Miyoshi Masahiro、Kikuchi Masayuki、et al
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 1-9

    • DOI

      10.3389/fmicb.2017.01513

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Heat shock protein 90 (HSP90)は風疹ウイルスの複製に関与する2017

    • Author(s)
      坂田真史、加藤大志、大槻紀之、岡本貴世子、竹田誠、森嘉生
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 風疹ウイルスは異なる2種類の方法で細胞へ吸着する2017

    • Author(s)
      森嘉生、大槻紀之、坂田真史、岡本貴世子、竹田誠
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Pseudotype VSV infection mediated by the rubella virus glycoprotein is restricted at the entry step in lymphoid cells2016

    • Author(s)
      坂田真史、谷秀樹、大槻紀之、岡本貴世子、安楽正輝、竹田誠、森嘉生
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of a small-molecule chemical compound inhibitor for hemagglutination and infection of rubella virus2016

    • Author(s)
      安楽正輝、松永智子、坂田真史、宮川敬、工藤あゆみ、大槻紀之、岡本貴世子、竹田誠、森嘉生、梁明秀
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Sphingomyelin of host cell plays an important role in the rubella virus infection2015

    • Author(s)
      大槻紀之、坂田真史、花田賢太郎、岡本貴世子、安楽正輝、竹田誠、森嘉生
    • Organizer
      第63回日本ウイルス学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of pseudotyped VSV bearing rubella virus envelope proteins2015

    • Author(s)
      坂田真史、谷秀樹、岡本貴世子、大槻紀之、安楽正輝、永井美智、竹田誠、森嘉生
    • Organizer
      第63回日本ウイルス学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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