| Project/Area Number |
15K08526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ADACHI Takahiro 東京医科歯科大学, 難治疾患研究所, 准教授 (50222625)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | イメージング / カルシウムシグナル / B細胞 / シグナル伝達 / 未病 / アレルギー / 自己免疫 / 生体イメージング / カルシウムシグナリング / 細胞内シグナル / 腸炎 / T細胞 / バイオセンサー / 自己免疫疾患 |
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| Outline of Final Research Achievements |
Calcium ion (Ca2+) signaling is a typical phenomenon mediated through immune receptors, and it is important for their biological activities. To analyze their signaling together with their in vivo dynamics, we generated a stable transgenic mouse line with the calcium indicator Yellow Cameleon 3.60 (YC3.60). We obtained mice with the specific YC3.60 expression in immune cells. We established five-dimensional (5D) (x, y, z, time, Ca2+) intravital imaging of various lymphoid tissues including the spleen, bone marrow and Peyer’s patches.Furthermore, in autoimmune-prone model mice, Ca2+ fluxes were augmented, although they did not induce autoimmune disease. Intravital imaging of Ca2+ signaling in lymphocytes may improve assessment of the risk of autoimmune diseases in model animals. These results suggest that intravital imaging of Ca2+ signaling may enable to evaluate immune responses under pathological and physiological conditions.
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