Visualizatiion of immune responses by 5D intravital imaging
| Project/Area Number |
15K08526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ADACHI Takahiro 東京医科歯科大学, 難治疾患研究所, 准教授 (50222625)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | イメージング / カルシウムシグナル / B細胞 / シグナル伝達 / 未病 / アレルギー / 自己免疫 / 生体イメージング / カルシウムシグナリング / 細胞内シグナル / 腸炎 / T細胞 / バイオセンサー / 自己免疫疾患 |
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| Outline of Final Research Achievements |
Calcium ion (Ca2+) signaling is a typical phenomenon mediated through immune receptors, and it is important for their biological activities. To analyze their signaling together with their in vivo dynamics, we generated a stable transgenic mouse line with the calcium indicator Yellow Cameleon 3.60 (YC3.60). We obtained mice with the specific YC3.60 expression in immune cells. We established five-dimensional (5D) (x, y, z, time, Ca2+) intravital imaging of various lymphoid tissues including the spleen, bone marrow and Peyer’s patches.Furthermore, in autoimmune-prone model mice, Ca2+ fluxes were augmented, although they did not induce autoimmune disease. Intravital imaging of Ca2+ signaling in lymphocytes may improve assessment of the risk of autoimmune diseases in model animals. These results suggest that intravital imaging of Ca2+ signaling may enable to evaluate immune responses under pathological and physiological conditions.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] B cell activation in the cecal patches during the development of an experimental colitis model.2018
Author(s)
Taro Watabe, Takashi Nagaishi, Naoya Tsugawa, Yudai Kojima, Nisha Jose, Akinori Hosoya, Michio Onizawa, Yasuhiro Nemoto, Shigeru Oshima, Tetsuya Nakamura, Hajime Karasuyama, Takahiro Adachi, Mamoru Watanabe
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Journal Title
Biochem Biophys Res Commun.
Volume: 496
Issue: 2
Pages: 367-373
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Visualization of Probiotic-Mediated Ca2+ Signaling in Intestinal Epithelial Cells In Vivo.2016
Author(s)
*Adachi T, Kakuta S, Aihara Y, Kamiya T, Watanabe Y, Osakabe N, Hazato N, Miyawaki A, Yoshikawa S, Usami T, Karasuyama H, Kimoto-Nira H, *Hirayama K, *Tsuji NM.
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Journal Title
Front Immunol.
Volume: 7
Pages: 601-601
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset.2016
Author(s)
Yoshikawa S, Usami T, Kikuta J, Ishii M, Sasano T, Sugiyama K, Furukawa T, Nakasho E, Takayanagi H, Tedder TF, Karasuyama H, Miyawaki A, Adachi T.
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Journal Title
Sci Rep. 2016 Jan 6;6:18738.
Volume: 6
Issue: 1
Pages: 18738-18738
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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