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Relation between the distribution of the stiffness of the LDL and age

Research Project

Project/Area Number 15K08607
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionHokkaido University

Principal Investigator

TAKEDA SEIJI  北海道大学, 保健科学研究院, 准教授 (80374726)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords低比重リポ蛋白質 / 硬さ / 酵素 / 加齢 / 脂質 / 年齢 / 低比重リポタンパク質 / 酸化 / 原子間力顕微鏡
Outline of Final Research Achievements

Oxidation of low-density lipoproteins (LDLs) is one of the risk factor for developing cardiovascular disease. Recently, we reported that the elastic modulus of oxidized LDLs decreased with increasing oxidation level of the LDL. However, the mechanisms of the decrease of the elastic modulus have not been well investigated. We thought that the structure change of the LDL might contributed to the decrease. This study measured the elastic modulus of LDLs before and after proteases, phospholipase A2. Although the enzyme treatment did not change the elastic modulus, treatment with PLA2 decreased the elastic modulus. Results suggest that structure change of the lipid structure of the LDL might contribute to the decreasing of the elastic modulus. We also investigated the relation between the age and the distribution of the LDL. Although detail more work will be required, we found that the elastic modulus tend to increase with increasing the age.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy2018

    • Author(s)
      Seiji Takeda, Toshihiro Sakurai, Shu-Ping Hui, Hirotoshi Fuda, Hitoshi Chiba
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] LDLの硬さと加齢について2018

    • Author(s)
      武田晴治、櫻井俊宏、惠淑萍、千葉仁志
    • Organizer
      第58回日本臨床化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effect of enzymes treatment on physical properties of low-density lipoprotein2017

    • Author(s)
      Seiji Takeda, Agus Subagyo, Shu-Ping Hui, Hirotoshi Fuda, Kazuhisa Sueoka, Hitoshi Chiba
    • Organizer
      第55回日本生物物理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] LDLの酸化および酵素処理が硬さに与える影響について2017

    • Author(s)
      武田晴治、Agus Subagyo, 布田博敏、惠淑萍、末岡和久、千葉仁志
    • Organizer
      第57回日本臨床化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸化還元電位および硬さ分布からLDLの酸化状態を評価2017

    • Author(s)
      武田晴治、Agus Subagyo, 布田博敏、惠淑萍、末岡和久、千葉仁志
    • Organizer
      第57回日本臨床化学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Physical properties of low-density lipoprotein after oxidation or enzyme treatment2016

    • Author(s)
      Seiji Takeda, Agus Subagyo, Shu-Ping Hui, Hirotoshi Fuda, Rojeet Shrestha, Kazuhisa Sueoka and Hitoshi Chiba
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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