Quantitative Analysis of Lipid Species in Chronic Kidney Disease using Imaging Mass Spectrometry

• Project/Area Number: 15K08608
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hokkaido University
• Principal Investigator: Hayasaka Takahiro 北海道大学, 医学研究院, 特任助教 (90415927)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 非アルコール性脂肪性肝炎 / 慢性腎臓病 / イメージング質量分析 / カルジオリピン / 質量分析イメージング / 脂質 / 抗酸化物質 / LC-MS / アポトーシス

Outline of Final Research Achievements

Non-alcoholic steatohepatitis (NASH) is drived from metabolic syndrome and simultaneously have a risk to complicate chronic kidney disease. In this study, imaging mass spectrometry (IMS) and conventional liquid-chromatography mass spectrometry (LC/MS) were used for analysis of lipid species on the kidney in NASH model mice. In the results, IMS revealed the specific distribution of molecular species at m/z 1,400-1,500 on the cortex of kidney section in NASH model mice and LC/MS identified these molecular species as cardiolipin with different fatty acids. Cardiolipin is known as a target by oxidative stress because of having four polyunsaturated fatty acids. The comparison with human pathologic specimen about cardiolipn as a molecular marker would be useful for a novel development of treatment and drug.

Research Products

• [Journal Article] The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma (2017)
  • Author(s): Ishikawa S, Tateya I, Hayasaka T, Shinriki S, Masaki N, Hirano S, Kitamura M, Muto M, Morita S, Setou M, Ito J.
  • Journal Title: Biomed Res Int Volume: 2017 Pages: 1-10
  • DOI: 10.1155/2017/5387913
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Different desmin peptides are distinctly deposited in cytoplasmic aggregations and cytoplasm of desmin-related cardiomyopathy patients (2017)
  • Author(s): Shintani-Domoto Y, Hayasaka T, Maeda D, Masaki N, Ito TK, Sakuma K, Tanaka M, Kabashima K, Takei S, Setou M, Fukayama M.
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Volume: S1570-9639 Pages: 30048-1
  • DOI: 10.1016/j.bbapap.2017.03.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Decreased 16:0/20:4-phosphatidylinositol level in the post-mortem prefrontal cortex of elderly patients with schizophrenia (2017)
  • Author(s): Matsumoto J, Nakanishi H, Kunii Y, Sugiura Y, Yuki D, Wada A, Hino M, Niwa SI, Kondo T, Waki M, Hayasaka T, Masaki N, Akatsu H, Hashizume Y, Yamamoto S, Sato S, Sasaki T, Setou M, Yabe H
  • Journal Title: Sci Rep Volume: 7 Issue: 7 Pages: 1-11
  • DOI: 10.1038/srep45050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Imaging Mass Spectrometry Reveals Decrease of Cardiolipin in the Kidney of NASH Model Mice (2016)
  • Author(s): Takahiro Hayasaka, Hirotoshi Fuda, Shu-Ping Hui, and Hitoshi Chiba
  • Journal Title: Analytical Sciences Volume: 33
  • NAID: 130005143829
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 質量分析イメージングの機能性食品成分研究への展開 (2015)
  • Author(s): 早坂孝宏
  • Organizer: 北海道バイオ産業クラスター・フォーラム
  • Place of Presentation: 札幌アスペンホテル（北海道札幌市）
  • Year and Date: 2015-11-18
  • Invited
• [Presentation] NASHモデルマウス腎組織の質量分析イメージング解析 (2015)
  • Author(s): 早坂孝宏、布田博敏、惠淑萍、千葉仁志
  • Organizer: 第22回日本未病システム学会学術総会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-10-11
• [Presentation] NASHモデルマウス腎組織における代謝変動の質量分析イメージングによる可視化 (2015)
  • Author(s): 早坂孝宏、布田博敏、惠淑萍、千葉仁志
  • Organizer: 遺伝子栄養学研究会
  • Place of Presentation: 北広島クラッセホテル（北海道新広島市）
  • Year and Date: 2015-09-04
• [Presentation] Imaging Mass Spectrometry Reveals the Decrease of Cardiolipin on Kidney of NASH model Mouse (2015)
  • Author(s): Takahiro Hayasaka, Hirotoshi Fuda, Shu-Ping Hui, Hitoshi Chiba
  • Organizer: 63rd Conference on Mass Spectrometry and allied topics
  • Place of Presentation: St. Louis (USA)
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Remarks] 高度脂質分析ラボラトリー
  • URL: http://www.hs.hokudai.ac.jp/innovation/lab1.html