Quantitative Analysis of Lipid Species in Chronic Kidney Disease using Imaging Mass Spectrometry
Project/Area Number |
15K08608
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Hokkaido University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 非アルコール性脂肪性肝炎 / 慢性腎臓病 / イメージング質量分析 / カルジオリピン / 質量分析イメージング / 脂質 / 抗酸化物質 / LC-MS / アポトーシス |
Outline of Final Research Achievements |
Non-alcoholic steatohepatitis (NASH) is drived from metabolic syndrome and simultaneously have a risk to complicate chronic kidney disease. In this study, imaging mass spectrometry (IMS) and conventional liquid-chromatography mass spectrometry (LC/MS) were used for analysis of lipid species on the kidney in NASH model mice. In the results, IMS revealed the specific distribution of molecular species at m/z 1,400-1,500 on the cortex of kidney section in NASH model mice and LC/MS identified these molecular species as cardiolipin with different fatty acids. Cardiolipin is known as a target by oxidative stress because of having four polyunsaturated fatty acids. The comparison with human pathologic specimen about cardiolipn as a molecular marker would be useful for a novel development of treatment and drug.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma2017
Author(s)
Ishikawa S, Tateya I, Hayasaka T, Shinriki S, Masaki N, Hirano S, Kitamura M, Muto M, Morita S, Setou M, Ito J.
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Journal Title
Biomed Res Int
Volume: 2017
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Different desmin peptides are distinctly deposited in cytoplasmic aggregations and cytoplasm of desmin-related cardiomyopathy patients2017
Author(s)
Shintani-Domoto Y, Hayasaka T, Maeda D, Masaki N, Ito TK, Sakuma K, Tanaka M, Kabashima K, Takei S, Setou M, Fukayama M.
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Journal Title
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Volume: S1570-9639
Pages: 30048-1
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Decreased 16:0/20:4-phosphatidylinositol level in the post-mortem prefrontal cortex of elderly patients with schizophrenia2017
Author(s)
Matsumoto J, Nakanishi H, Kunii Y, Sugiura Y, Yuki D, Wada A, Hino M, Niwa SI, Kondo T, Waki M, Hayasaka T, Masaki N, Akatsu H, Hashizume Y, Yamamoto S, Sato S, Sasaki T, Setou M, Yabe H
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Journal Title
Sci Rep
Volume: 7
Issue: 7
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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