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Quantitative Analysis of Lipid Species in Chronic Kidney Disease using Imaging Mass Spectrometry

Research Project

Project/Area Number 15K08608
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionHokkaido University

Principal Investigator

Hayasaka Takahiro  北海道大学, 医学研究院, 特任助教 (90415927)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords非アルコール性脂肪性肝炎 / 慢性腎臓病 / イメージング質量分析 / カルジオリピン / 質量分析イメージング / 脂質 / 抗酸化物質 / LC-MS / アポトーシス
Outline of Final Research Achievements

Non-alcoholic steatohepatitis (NASH) is drived from metabolic syndrome and simultaneously have a risk to complicate chronic kidney disease. In this study, imaging mass spectrometry (IMS) and conventional liquid-chromatography mass spectrometry (LC/MS) were used for analysis of lipid species on the kidney in NASH model mice. In the results, IMS revealed the specific distribution of molecular species at m/z 1,400-1,500 on the cortex of kidney section in NASH model mice and LC/MS identified these molecular species as cardiolipin with different fatty acids. Cardiolipin is known as a target by oxidative stress because of having four polyunsaturated fatty acids. The comparison with human pathologic specimen about cardiolipn as a molecular marker would be useful for a novel development of treatment and drug.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report

Research Products

(9 results)

All 2017 2016 2015 Other

All Journal Article Presentation Remarks

  • [Journal Article] The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma2017

    • Author(s)
      Ishikawa S, Tateya I, Hayasaka T, Shinriki S, Masaki N, Hirano S, Kitamura M, Muto M, Morita S, Setou M, Ito J.
    • Journal Title

      Biomed Res Int

      Volume: 2017 Pages: 1-10

    • DOI
      10.1155/2017/5387913
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Different desmin peptides are distinctly deposited in cytoplasmic aggregations and cytoplasm of desmin-related cardiomyopathy patients2017

    • Author(s)
      Shintani-Domoto Y, Hayasaka T, Maeda D, Masaki N, Ito TK, Sakuma K, Tanaka M, Kabashima K, Takei S, Setou M, Fukayama M.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

      Volume: S1570-9639 Pages: 30048-1

    • DOI
      10.1016/j.bbapap.2017.03.006
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Decreased 16:0/20:4-phosphatidylinositol level in the post-mortem prefrontal cortex of elderly patients with schizophrenia2017

    • Author(s)
      Matsumoto J, Nakanishi H, Kunii Y, Sugiura Y, Yuki D, Wada A, Hino M, Niwa SI, Kondo T, Waki M, Hayasaka T, Masaki N, Akatsu H, Hashizume Y, Yamamoto S, Sato S, Sasaki T, Setou M, Yabe H
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 7 Pages: 1-11

    • DOI
      10.1038/srep45050
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Imaging Mass Spectrometry Reveals Decrease of Cardiolipin in the Kidney of NASH Model Mice2016

    • Author(s)
      Takahiro Hayasaka, Hirotoshi Fuda, Shu-Ping Hui, and Hitoshi Chiba
    • Journal Title

      Analytical Sciences

      Volume: 33

    • NAID
      130005143829
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 質量分析イメージングの機能性食品成分研究への展開2015

    • Author(s)
      早坂孝宏
    • Organizer
      北海道バイオ産業クラスター・フォーラム
    • Place of Presentation
      札幌アスペンホテル(北海道札幌市)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] NASHモデルマウス腎組織の質量分析イメージング解析2015

    • Author(s)
      早坂孝宏、布田博敏、惠淑萍、千葉仁志
    • Organizer
      第22回日本未病システム学会学術総会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-10-11
    • Related Report
      2015 Research-status Report
  • [Presentation] NASHモデルマウス腎組織における代謝変動の質量分析イメージングによる可視化2015

    • Author(s)
      早坂孝宏、布田博敏、惠淑萍、千葉仁志
    • Organizer
      遺伝子栄養学研究会
    • Place of Presentation
      北広島クラッセホテル(北海道新広島市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
  • [Presentation] Imaging Mass Spectrometry Reveals the Decrease of Cardiolipin on Kidney of NASH model Mouse2015

    • Author(s)
      Takahiro Hayasaka, Hirotoshi Fuda, Shu-Ping Hui, Hitoshi Chiba
    • Organizer
      63rd Conference on Mass Spectrometry and allied topics
    • Place of Presentation
      St. Louis (USA)
    • Year and Date
      2015-05-31
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 高度脂質分析ラボラトリー

    • URL
      http://www.hs.hokudai.ac.jp/innovation/lab1.html
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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