| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
It has been estimated that autoimmunity is involved in the pathogenesis for severe dyslipidemia with unknown origin, but its diagnosis is not easy. In this study, we have developed a system to detect the anti-apolipoprotein (apo) C-II autoantibodies, which lead to severe hypertriglyceridemia. As a first screening test, we measured the serum anti-apo C-II autoantibody levels against the synthetic partial peptides of apo C-II by ELISA (Enzyme-Linked ImmunoSorbent Assay). Among the five selected patients of hypertriglyceridemia with high autoantibody levels, we could identify the autoantibodies associated with the endogenous apo C-II by immunoprecipitation/immunoblot method in two patients. Also, we found that the serum levels of IgA class autoantibody might be related with the stage of diabetic nephropathy.
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