Development of diagnostic methods for autoimmune dyslipidemia
Project/Area Number |
15K08613
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
|
Research Institution | Osaka University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
木原 進士 大阪大学, 医学系研究科, 教授 (20332736)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 自己免疫 / 脂質異常症 / 高中性脂肪血症 / 自己抗体 |
Outline of Final Research Achievements |
It has been estimated that autoimmunity is involved in the pathogenesis for severe dyslipidemia with unknown origin, but its diagnosis is not easy. In this study, we have developed a system to detect the anti-apolipoprotein (apo) C-II autoantibodies, which lead to severe hypertriglyceridemia. As a first screening test, we measured the serum anti-apo C-II autoantibody levels against the synthetic partial peptides of apo C-II by ELISA (Enzyme-Linked ImmunoSorbent Assay). Among the five selected patients of hypertriglyceridemia with high autoantibody levels, we could identify the autoantibodies associated with the endogenous apo C-II by immunoprecipitation/immunoblot method in two patients. Also, we found that the serum levels of IgA class autoantibody might be related with the stage of diabetic nephropathy.
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Report
(4 results)
Research Products
(3 results)