| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Comprehensive quantitative analysis of glycolipids targeting molecular species has not studied fully yet because of the diverse structures of sugar and ceramide moieties of glycosphingolipids(GLS). To establish a method for comprehensive quantitative analysis of GSLs by LC-ESI-MS, the investigation of separation of GSLs using a chiral column has been done. IF-3 chiral column has an advantage in separating GSLs according to not only their ceramide types but also the differences in sugar moieties. Next, the quantitative analysis of GLS in HeLa cells,and also sulfatide standards from porcine brain by multiple reaction monitoring mode. IF-3 was able to detect GSLs present in trace amounts in the total lipid extracts from biological samples.
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