Establishment of glycosphingolipid analysis in biological samples and application in clinical diagnosis of inherited metabolic disorders
| Project/Area Number |
15K08625
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 糖脂質 / 質量分析 / 先天代謝異常症 / スフィンゴ糖脂質 / LC-MS / キラルカラム / スフィンゴリピドーシス / LC-ESI-MS / ペルオキシソーム病 |
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| Outline of Final Research Achievements |
Comprehensive quantitative analysis of glycolipids targeting molecular species has not studied fully yet because of the diverse structures of sugar and ceramide moieties of glycosphingolipids(GLS). To establish a method for comprehensive quantitative analysis of GSLs by LC-ESI-MS, the investigation of separation of GSLs using a chiral column has been done. IF-3 chiral column has an advantage in separating GSLs according to not only their ceramide types but also the differences in sugar moieties. Next, the quantitative analysis of GLS in HeLa cells,and also sulfatide standards from porcine brain by multiple reaction monitoring mode. IF-3 was able to detect GSLs present in trace amounts in the total lipid extracts from biological samples.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] LC-MS/MSによるAbcd1ノックアウトマウス脳の極長鎖脂肪酸含有リン脂質分子種の解析と糖脂質解析法の検討2016
Author(s)
笠原美那実, 笠松瞳, 豊島永莉, 堀口静香前田絵里加,南佳那, 渡邉里奈, 濱弘太郎,藤原優子, 下澤伸行,守田雅志, 今中常雄,横山和明
Organizer
日本薬学会 136年会
Place of Presentation
パシフィコ横浜
Year and Date
2016-03-27
Related Report
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