Nobel therapeutic strategy for carbapenem-resistant Acinetobacter spp.

• Project/Area Number: 15K08638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tohoku University
• Principal Investigator: Endo Shiro 東北大学, 医学系研究科, 大学院非常勤講師 (40614491)
• Co-Investigator(Kenkyū-buntansha): 矢野 寿一 奈良県立医科大学, 医学部, 教授 (20374944) ; 中野 竜一 奈良県立医科大学, 医学部, 講師 (80433712)
• Research Collaborator: SUZUKI Yuki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Acinetobacter / アシネトバクター / カルバペネマーゼ / スクリーニング検査

Outline of Final Research Achievements

The prevalence of drug-resistant Acinetobacter spp. has increased and is a serious concern worldwide. The aims of this study were to clarify species-level identification, antibiotic susceptibilities, and mechanisms of carbapenem resistant of Acinetobacter spp. using 60 Acinetobacter clinical isolates from sputum specimens in Japan. Genomic species were identified by sequencing of the rpoB gene. MICs were determined by the agar dilution method following CLSI guidelines (M100-S25). Carbapenemase genes and the genetic environment were investigated by PCR and DNA sequencing. Five Acinetobacter spp. isolates were resistant to carbapenem and encoded the IMP-34 gene. These five strains harboured a class 1 integron and carried a gene cassettes. In this study, we described the distribution and species level identification of Acinetobacter spp. isolated from sputum specimens in Japan. These included IMP-34 producers, which is the first detection of IMP-34 among Acinetobacter spp.

Research Products

• [Journal Article] Suitability of the Carbapenem Inactivation Method (CIM) to Detect IMP Metallo-β-lactamase-Producing Enterobacteriaceae. (2017)
  • Author(s): K Saito, R Nakano, Y Suzuki, A Nakano, Y Ogawa, S Yonekawa, S Endo, F Mizuno, K Kasahara, K Mikasa, M Kaku, H Yano
  • Journal Title: Journal of Clinical Microbiology Volume: 55 Issue: 4 Pages: 1220-1222
  • DOI: 10.1128/jcm.02275-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loop-mediated isothermal amplification: Rapid and sensitive detection of the antibiotic resistance gene ISAba1-blaOXA-51-like in Acinetobacter baumannii (2016)
  • Author(s): Xiaoqin Mu, Ryuichi Nakano, Akiyo Nakano, Tsuneyuki Ubagai, Takane Kikuchi-Ueda, Shigeru Tansho-Nagakawa, Hirotoshi Kikuchi, Go Kamoshida, Shiro Endo, Hisakazu Yano, Yasuo Ono
  • Journal Title: Journal of Microbiological Methods Volume: 121 Pages: 36-40
  • DOI: 10.1016/j.mimet.2015.12.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Molecular epidemiology and mechanisms of carbapenem-resistant Acinetobacter spp. clinical isolates from sputum specimen in Japan (2017)
  • Author(s): Y. Suzuki, S. Endo, R. Nakano, K. Saito, A. Nakano, F. Mizuno, H. Yano, M. Kaku
  • Organizer: 27th ECCMID, Vienna, Austria, 2017
  • Int'l Joint Research