| Project/Area Number |
15K08640
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小島 寛 筑波大学, 医学医療系, 教授 (10225435)
島居 徹 筑波大学, 医学医療系, 教授 (80235613)
玉木 義雄 筑波大学, 医学医療系, 教授 (60188422)
沖 明典 筑波大学, 医学医療系, 教授 (60334067)
鈴木 久史 筑波大学, 医学医療系, 講師 (40750740)
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| Research Collaborator |
INADA Katsushige
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 原発不明がん / マイクロRNA / 原発組織 / ホルマリン固定パラフィン包埋 / リンパ節 / 内在性コントロール遺伝子 / リファレンス |
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| Outline of Final Research Achievements |
To assess the primary tumor of metastatic lesion, we investigated microRNA (miRNA) expression patterns using formalin-fixed, paraffin-embedded (FFPE) lymph node tissues. Initial analysis of 31 samples from 11 types of tumor resulted that the primary site was correctly predicted in 71 %. To achieve higher accuracy, we noticed that an endogenous reference gene for miRNA expression analysis had not been identified in FFPE lymph node with metastatic cancer. A suitable endogenous reference gene should be evaluated to avoid misinterpretation of data and identify true changes in miRNA expression levels. Finally, we found that the combination of three miRNAs, miR-24/miR-103a/let-7a, could be used as a suitable endogenous reference in a lymph node with metastatic cancer.
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