Global analysis of cancer-tissue-derived microRNA to improve treatment outcome of cancer of unknown primary

• Project/Area Number: 15K08640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Tsukuba
• Principal Investigator: OKOSHI Yasushi 筑波大学, 医学医療系, 准教授 (10400673)
• Co-Investigator(Kenkyū-buntansha): 小島 寛 筑波大学, 医学医療系, 教授 (10225435) ; 島居 徹 筑波大学, 医学医療系, 教授 (80235613) ; 玉木 義雄 筑波大学, 医学医療系, 教授 (60188422) ; 沖 明典 筑波大学, 医学医療系, 教授 (60334067) ; 鈴木 久史 筑波大学, 医学医療系, 講師 (40750740)
• Research Collaborator: INADA Katsushige
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 原発不明がん / マイクロRNA / 原発組織 / ホルマリン固定パラフィン包埋 / リンパ節 / 内在性コントロール遺伝子 / リファレンス

Outline of Final Research Achievements

To assess the primary tumor of metastatic lesion, we investigated microRNA (miRNA) expression patterns using formalin-fixed, paraffin-embedded (FFPE) lymph node tissues. Initial analysis of 31 samples from 11 types of tumor resulted that the primary site was correctly predicted in 71 %. To achieve higher accuracy, we noticed that an endogenous reference gene for miRNA expression analysis had not been identified in FFPE lymph node with metastatic cancer. A suitable endogenous reference gene should be evaluated to avoid misinterpretation of data and identify true changes in miRNA expression levels. Finally, we found that the combination of three miRNAs, miR-24/miR-103a/let-7a, could be used as a suitable endogenous reference in a lymph node with metastatic cancer.

Research Products

• [Journal Article] Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue (2018)
  • Author(s): Inada K, Okoshi Y, Cho-Isoda Y, Ishiguro S, Suzuki H, Oki A, Tamaki Y, Shimazui T, Saito H, Hori M, Iijima T, Kojima H
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 5918-5927
  • DOI: 10.1038/s41598-018-24338-7
  • NAID: 120007134247
  • Peer Reviewed / Open Access
• [Presentation] Reference gene selection for microRNA quantitation in lymph node tissue (2017)
  • Author(s): Inada K, Okoshi Y, Cho Y, Iijima T, Hori M, Kojima H
  • Organizer: 第76回 日本癌学会学術総会