Novel rapid quantification method of viable bacteria in a septic blood sample can produce an effective biomarker for monitoring patient care
| Project/Area Number |
15K08642
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
NIIMI HIDEKI 富山大学, 大学院医学薬学研究部(医学), 准教授 (50401865)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北島 勲 富山大学, 大学院医学薬学研究部(医学), 教授 (50214797)
野手 良剛 富山大学, 附属病院, 主事 (60377364)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 敗血症 / 感染症 / 遺伝子検査 / バイオマーカー / 迅速検査 / 起炎菌の定量 / 起炎菌の同定 / Viability PCR |
|---|
| Outline of Final Research Achievements |
Severe sepsis is a common and frequently fetal condition, but current biomarkers do not always reflect the severity of sepsis at a particular point in time. Here we show the development of a novel rapid identification and quantification method of unknown bacteria in a clinical sample using the original technology. We identified and quantified bacteria in septic blood samples, and the quantification results were correlated with the severity of microbial infection . We subsequently examined the time-dependent changes of blood bacterial concentration, and found that the time-dependent changes of blood bacterial concentration were not correlated with those of BT, WBC, CRP, IL-6, and Presepsin. In particular, 24 to 72 hours after antibiotic treatments, blood bacterial concentrations were dramatically decreased. In conclusion, blood bacterial concentration would be useful as a novel biomarker not only to estimate the severity of microbial infection but to monitor the therapeutic effect.
|
Report
(4 results)
Research Products
(37 results)
-
[Journal Article] Sludge reflects intra-amniotic inflammation with or without microorganisms.2018
Author(s)
Yoneda N, Yoneda S, Niimi H, Ito M, Fukuta K, Ueno T, Ito M, Shiozaki A, Kigawa M, Kitajima I, Saito S.
-
Journal Title
Am J Reprod Immunol.
Volume: 79(2)
Issue: 2
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] 17OHP-C in patients with spontaneous preterm labor and intact membranes: is there an effect according to the presence of intra-amniotic inflammation?2018
Author(s)
Yoneda S, Yoneda N, Shiozaki A, Yoshino O, Ueno T, Niimi H, Kitajima I, Tamura K, Kawasaki Y, Makimoto M, Yoshida T, Saito S.
-
Journal Title
Am J Reprod Immunol.
Volume: 79
Issue: 3
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Antibiotic Therapy Increases the Risk of Preterm Birth in Preterm Labor without Intra-Amniotic Microbes, but may Prolong the Gestation Period in Preterm Labor with Microbes, Evaluated by Rapid and High-Sensitive PCR System2016
Author(s)
Yoneda S, Shiozaki A, Yoneda N, Ito M, Shima T, Fukuda K, Ueno T, Niimi H, Kitajima I, Kigawa M, Saito S
-
Journal Title
American Journal of Reproductive Immunology
Volume: 75(4)
Issue: 4
Pages: 440-50
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Melting temperature mapping method: A novel method for identification of unknown pathogenic microorganisms within three hours of sample of collection.2015
Author(s)
Niimi H, Ueno T, Hayashi S, Abe A, Tsurue T, Mori M, Tabata H, Minami H, Goto M, Akiyama M, Yamamoto Y, Saito S, Kitajima I
-
Journal Title
Scientific Reports.
Volume: 5
Issue: 1
Pages: 12543-12543
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor2015
Author(s)
Yoneda N, Yoneda S, Niimi H, Ueno T, Hayashi S, Ito M, Shiozaki A, Urushiyama D, Hata K, Suda W, Hattori M, Kigawa M, Kitajima I, Saito S.
-
Journal Title
American Journal of Reproductive Immunology
Volume: 75(2)
Issue: 2
Pages: 112-25
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
