| Project/Area Number |
15K08642
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
NIIMI HIDEKI 富山大学, 大学院医学薬学研究部(医学), 准教授 (50401865)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北島 勲 富山大学, 大学院医学薬学研究部(医学), 教授 (50214797)
野手 良剛 富山大学, 附属病院, 主事 (60377364)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 敗血症 / 感染症 / 遺伝子検査 / バイオマーカー / 迅速検査 / 起炎菌の定量 / 起炎菌の同定 / Viability PCR |
|---|
| Outline of Final Research Achievements |
Severe sepsis is a common and frequently fetal condition, but current biomarkers do not always reflect the severity of sepsis at a particular point in time. Here we show the development of a novel rapid identification and quantification method of unknown bacteria in a clinical sample using the original technology. We identified and quantified bacteria in septic blood samples, and the quantification results were correlated with the severity of microbial infection . We subsequently examined the time-dependent changes of blood bacterial concentration, and found that the time-dependent changes of blood bacterial concentration were not correlated with those of BT, WBC, CRP, IL-6, and Presepsin. In particular, 24 to 72 hours after antibiotic treatments, blood bacterial concentrations were dramatically decreased. In conclusion, blood bacterial concentration would be useful as a novel biomarker not only to estimate the severity of microbial infection but to monitor the therapeutic effect.
|
