| Project/Area Number |
15K08650
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
伊藤 利洋 奈良県立医科大学, 医学部, 教授 (00595712)
石谷 昭子 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Obayashi Chiho 奈良県立医科大学, 医学部, 教授 (90223940)
Nakai Tokiko 奈良県立医科大学, 医学部, 助教 (00619538)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 免疫染色 / 腫瘍 / 診断 / HLA-F / 大腸癌 / ホルマリン標本 / 低分化癌 / 悪性度 |
|---|
| Outline of Final Research Achievements |
We have investigated the HLA-F expression on tumor tissues to develop the new method for the tumor diagnosis using HLA-F. HLA-F is one of HLA class I molecules, but it has low polymorphism and has restricted expression pattern. The staining method of HLA-F was optimized for the formalin fixed-paraffin embedded tumor tissues. HLA-F expression was detected all poorly-differentiated colon cancer, which we have tested. Especially, the strong HLA-F expression was detected on 75% of poorly-differentiated colon cancer and 26% of moderately- or well-differentiated colon cancer. On the analysis of HLA-F mRNA on tumor cell lines, HLA-F mRNA was upregulated following with tumor malignancy. These results indicated that HLA-F expression on tumor was associated with tumor malignancy. However we need more investigation to show clearly the association between HLA-F and tumor progression and to show utility of new system for tumor diagnosis using HLA-F.
|
