• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The development of new pathologic diagnosis system using HLA-F expression on tumor

Research Project

Project/Area Number 15K08650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionNara Medical University

Principal Investigator

Ouji-Sageshima Noriko  奈良県立医科大学, 医学部, 助教 (30398432)

Co-Investigator(Kenkyū-buntansha) 伊藤 利洋  奈良県立医科大学, 医学部, 教授 (00595712)
石谷 昭子  奈良県立医科大学, 医学部, 非常勤講師 (40112544)
Co-Investigator(Renkei-kenkyūsha) Obayashi Chiho  奈良県立医科大学, 医学部, 教授 (90223940)
Nakai Tokiko  奈良県立医科大学, 医学部, 助教 (00619538)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords免疫染色 / 腫瘍 / 診断 / HLA-F / 大腸癌 / ホルマリン標本 / 低分化癌 / 悪性度
Outline of Final Research Achievements

We have investigated the HLA-F expression on tumor tissues to develop the new method for the tumor diagnosis using HLA-F. HLA-F is one of HLA class I molecules, but it has low polymorphism and has restricted expression pattern. The staining method of HLA-F was optimized for the formalin fixed-paraffin embedded tumor tissues. HLA-F expression was detected all poorly-differentiated colon cancer, which we have tested. Especially, the strong HLA-F expression was detected on 75% of poorly-differentiated colon cancer and 26% of moderately- or well-differentiated colon cancer. On the analysis of HLA-F mRNA on tumor cell lines, HLA-F mRNA was upregulated following with tumor malignancy. These results indicated that HLA-F expression on tumor was associated with tumor malignancy. However we need more investigation to show clearly the association between HLA-F and tumor progression and to show utility of new system for tumor diagnosis using HLA-F.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (7 results)

  • [Journal Article] Establishment of optimized ELISA system specific for HLA-G in body fluids.2016

    • Author(s)
      Ouji-Sageshima N, Geraghty DE, Ishitani A, Hatake K, Ito T
    • Journal Title

      HLA

      Volume: 88 Issue: 6 Pages: 293-299

    • DOI

      10.1111/tan.12919

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] sHLA-G and sHLA-I levels in follicular fluid are not associated with successful implantation.2016

    • Author(s)
      Ouji-Sageshima N, Yuui K, Nakanishi M, Takeda N, Odawara Y, Yamashita M, Iwayama H, Awai K, Hashimoto H, Geraghty DE, Ishitani A, Hatake K, Ito T
    • Journal Title

      J Reprod Immunol.

      Volume: 113 Pages: 16-21

    • DOI

      10.1016/j.jri.2015.10.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 腫瘍細胞株におけるHLA-E、-F、-G分子の発現2017

    • Author(s)
      王寺-下嶋 典子、石谷 昭子、Daniel E Geraghty、伊藤 利洋
    • Organizer
      第26回日本組織適合性学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The critical role of HLA class Ib proteins, HLA-E and HLA-F, expressed on tumor cells2017

    • Author(s)
      Ouji-Sageshima Noriko, Ishitani Akiko, Imakita Natsuko, Sonobe Shota, Kitbatake Masahiro, Daniel E Geraghty, Ito Toshihiro
    • Organizer
      The 46th Japanese Society for Immunology
    • Related Report
      2017 Annual Research Report
  • [Presentation] Does HLA-G in plasma from pregnant woman inhibit the rejection of fetus?2016

    • Author(s)
      Noriko OUJI-SAGESHIMA, Akiko ISHITANI, Daniel E Geraghty, Toshihiro ITO
    • Organizer
      第45回 日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
  • [Presentation] HLA-Gは体外受精卵培養上清および妊婦血漿等体液中に存在するのか?2016

    • Author(s)
      下嶋典子、Daniel E Geraghty、石谷昭子、伊藤利洋
    • Organizer
      第31回 日本生殖免疫学会総会・学術集会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 改良ELISA法による体外受精卵培養上清および妊婦血漿等体液中のHLA-Gの測定とその意義2016

    • Author(s)
      王寺-下嶋典子、Daniel E Geraghty、石谷昭子、伊藤利洋
    • Organizer
      第25回 日本組織適合性学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] The relation between the expression of both HLA-F and HLA-G on PBMC from pretransplant and posttransplant patients and the graft survival.2015

    • Author(s)
      Noriko Ouji-Sageshima, Daniel E Geraghty, Akiko Ishitani, Toshihiro Ito
    • Organizer
      日本免疫学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 移植患者末梢血における HLA-F、HLA-Gの発現解析―移植片生着との関連性について―」2015

    • Author(s)
      下嶋典子、貝森淳哉、市丸直嗣、吉澤淳、一戸辰夫、森井武志、長谷川淳、米田龍生、高原史郎、上本伸二、吉田克法、羽竹勝彦、伊藤利洋、Daniel E Geraghty、石谷昭子
    • Organizer
      第24回日本組織適合性学会大会(水戸)
    • Place of Presentation
      ホテルレイクビュー水戸
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi