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Molecular mechanisms of refractory leukemia cells in the bone marrow microenvironment

Research Project

Project/Area Number 15K08654
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionTokai University

Principal Investigator

MIYACHI Hayato  東海大学, 医学部, 教授 (20174196)

Research Collaborator DAMDINSUREN Anar  東海大学, 医学部, 研究員
NEMEKHBAATAR Lkhaasuren  東海大学, 医学部, 研究員
TSEVEGJAV Bayarbat  東海大学, 医学部, 研究員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords白血病 / 治療抵抗性 / 抗がん剤 / 骨髄微小環境 / FLT3-ITD / 遺伝子診断 / 急性骨髄性白血病 / 遺伝子変異 / チロシンキナーゼレセプター / 骨髄間質細胞 / 遺伝子
Outline of Final Research Achievements

This study was conducted to clarify molecular mechanisms of refractory leukemia cells in the bone marrow microenvironment. When FLT3-ITD positive leukemia cell lines (either exogenous or endogenous, K562 or MOLM-14, MV4:11, respectively) were cultured in the presence of components of extracellular matrix, such as fibronectin, of the bone marrow microenvironment, Ara-C resitance was significantly and specifically enhanced. Genes coding fibronectin or its receptor are increased in the expression at the interaction. These findings would be a part of molecular mechanisms of refractory leukemia cells in the bone marrow microenvironment after chemotherapy. The involved genes and signals would contributory to biomarkers relevant for diagnosis and monitoring the disease.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Prognostic value of genetic mutations in adolescent and young adults with acute myeloid leukemia2017

    • Author(s)
      Kuwatsuka Y、Asou N、Ohtake S、Miyawaki S、Miyazaki Y、Sakura T、Ozawa Y、Usui N、Kanamori H、Ito Y、Imai K、Suehiro Y、Kobayashi S、Kitamura K、Sakaida E、Ogawa S、Naoe T、Hayashi Y、Horibe K、Manabe A、Mizutani S、Adachi S、Kiyoi H et al.
    • Journal Title

      International Journal of Hematology

      Volume: 107 Issue: 2 Pages: 201-210

    • DOI

      10.1007/s12185-017-2340-z

    • NAID

      120006473552

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High event-free survival rate with minimum-dose-anthracycline treatment in childhood acute promyelocytic leukaemia: a nationwide prospective study by the Japanese Paediatric Leukaemia/Lymphoma Study Group.2016

    • Author(s)
      Takahashi H, Watanabe T, Kinoshita A, Yuza Y, Moritake H, Terui K, Iwamoto S, Nakayama H, Shimada A, Kudo K, Taki T, Yabe M, Matsushita H, Yamashita Y, Koike K, Ogawa A, Kosaka Y, Tomizawa D, Taga T, Saito AM, Horibe K, Nakahata T, Miyachi H, Tawa A, Adachi S.
    • Journal Title

      Br J Haematol.

      Volume: 印刷中 Issue: 3 Pages: 437-443

    • DOI

      10.1111/bjh.14068

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis to Estimate Genetic Variations in the Idarubicin-Resistant Derivative MOLT-3.2016

    • Author(s)
      Komiyama T, Ogura A, Hirokawa T, Zhijing M, Kamiguchi H, Asai S, Miyachi H, Kobayashi H.
    • Journal Title

      Int J Mol Sci.

      Volume: 18

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Erythrophagocytosis in T-cell type acute lymphoblastic leukaemia with near-tetraploidy.2016

    • Author(s)
      Watanabe A, Akahane K, Somazu S, Oshiro H, Goi K, Miyachi H, Kiyokawa N, Inukai T, Sugita K.
    • Journal Title

      J Clin Pathol.

      Volume: 69 Pages: 1129-1132

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] FLT3-ITD drives Ara-C resistance in leukemic cells via the induction of RUNX3.2015

    • Author(s)
      Damdinsuren A, Matsushita H, Ito M, Tanaka M, Jin G, Tsukamoto H, Asai S, Ando K, Miyachi H.
    • Journal Title

      Leukeimia Research

      Volume: 39 Issue: 12 Pages: 1405-13

    • DOI

      10.1016/j.leukres.2015.09.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The roles of the stromal cells in the chemotherapy resistance in AML with RTK mutation.2017

    • Author(s)
      Anar Damdinsuren, Satomi Asai, Hayato Miyachi
    • Organizer
      29th World Congress of World Association of Societies of Pathology and Laboratory Medicine
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fibronectin mediates Ara-C resistance in FLT3-ITD leukemia.2017

    • Author(s)
      ツェベグジャブ バヤルバット、ネメフバータール ラハースレン、ダムディンスレン アナラ、浅井 さとみ、宮地 勇人
    • Organizer
      第64回日本臨床検査医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Generation of the leukemic cell line models with common kit mutations2016

    • Author(s)
      A. Damdinsuren, H. Matsushita, S.Asai, H.Miyachi
    • Organizer
      63th annual meeting of Japanese Society of Laboratory Medicine
    • Place of Presentation
      Kobe international Conference Center (Kobe, Japan)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Differential expression analysis of proteome and transcriptome in FLT3-ITD positive leukemia cells2015

    • Author(s)
      Nemekhbaatar L,Damdinsuren A, Ito M, Tsukamoto H, Asai S, Matsushita H, Miyachi H.
    • Organizer
      第62回 日本臨床検査医学会学術集会
    • Place of Presentation
      岐阜都ホテル(岐阜県岐阜市)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
  • [Presentation] RUNX3 in FLT3-ITD-induced Ara-C resistance2015

    • Author(s)
      Damdinsuren A, Matsushita H, Ito M, Tanaka M, Jin G, Tsukamoto H, Asai S, Ando K, Miyachi, H.
    • Organizer
      第62回 日本臨床検査医学会学術集会
    • Place of Presentation
      岐阜都ホテル(岐阜県岐阜市)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
  • [Presentation] RUNX3 is prerequisite for FLT3-ITD-induced Ara-C resistance in leukemic cells2015

    • Author(s)
      Damdinsuren A, Matsushita H, Ito M, Tanaka M, Jin G, Tsukamoto H, Asai S, Ando K, Miyachi H.
    • Organizer
      第77回 日本血液学会学術集会
    • Place of Presentation
      ホテル日航金沢(石川県金沢市)
    • Year and Date
      2015-10-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative analysis of proteome and transcriptome in FLT3-ITD transducer leukemic cells2015

    • Author(s)
      Nemekhbaatar L, Damdinsuren A, Ito M, Tsukamoto H, Asai S, Matsushita H, Miyachi H.
    • Organizer
      第22回 日本遺伝子診療学会大会
    • Place of Presentation
      かながわ労働プラザ(神奈川県横浜市)
    • Year and Date
      2015-07-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Microarray gene expression profiling of the FLT3-ITD-transduced leukemic cells2015

    • Author(s)
      Damdinsuren A, Matsushita H, Ito M, Tanaka M, Jin G, Tsukamoto H, Asai S, Ando K, Miyachi H.
    • Organizer
      第22回 日本遺伝子診療学会大会
    • Place of Presentation
      かながわ労働プラザ(神奈川県横浜市)
    • Year and Date
      2015-07-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2021-03-11  

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