| Project/Area Number |
15K08673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤田 亜美 佐賀大学, 医学部, 准教授 (70336139)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | orexin A / orexin B / excitatory transmission / inhibitory transmission / inward current / spinal dorsal horn / pain / patch clamp / オレキシンA / オレキシンB / 脊髄後角 / 抑制性シナプス伝達 / GABA / グリシン / ラット / パッチクランプ法 / オレキシンAとオレキシンB / 視床下部ペプチド / グルタミン酸の自発放出促進 / 膜の脱分極 / 脊髄後角第II層 / 鎮痛 / 成熟ラット / オレキシン / グルタミン酸自発放出促進 / 脊髄膠様質 |
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| Outline of Final Research Achievements |
Cellular mechanisms underlying the antinociceptive effects of hypothalamic neuropeptides orexins (OXs) in the spinal dorsal horn have not been fully investigated. We examined how OXA and OXB affect spontaneous synaptic transmission in spinal substantia gelatinosa (SG) neurons, which play a pivotal role in regulating nociceptive transmission, by using the patch-clamp technique and adult rat spinal cord slices. As a result, we found out that OXA and OXB concentration-dependently produce a membrane depolarization and/or increased spontaneous L-glutamate release from nerve terminals, both of which actions lead to an increase in neuronal activity of SG neurons, resulting in enhancement by OXA of both glycinergic and GABAergic spontaneous inhibitory transmission and by OXB of only glycinergic transmission; these activities are mediated by orexin-1 and orexin-2 receptor activation, respectively. These actions of OXA and OXB could contribute to their antinociceptive effects.
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