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Development methods of isolation of novel anti-allodynic peptide from cerebral tissue of rats and methods of bioassay for anti-allodynic activity

Research Project

Project/Area Number 15K08676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pain science
Research InstitutionKyoto Tachibana University (2018)
University of Miyazaki (2015-2017)

Principal Investigator

IKEDA TETSUYA  京都橘大学, 健康科学部, 教授 (20264369)

Co-Investigator(Kenkyū-buntansha) 安部 博史  北海道医療大学, 心理科学部, 教授 (20344848)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords慢性疼痛 / 糖尿病性疼痛 / 神経因性疼痛 / APGWamide / アロディニア / 神経ペプチド / HPLC / 疼痛
Outline of Final Research Achievements

In preliminary experiment, a novel substance exhibited anti-allodynic activity has been isolated from cerebral tissue of mice (1000animals) in the first year of this investigation program. But the purified substance was too small amount to analyze that molecular structure. When we isolate anti-allodynic substances from rat cerebral, a great deal of rats beyond a schedule are needed. In generally, common neuropeptides and neuroactive substances are distributed in the brain of vertebrate. From the above reason, we decided to isolate APGWamide-like anti-allodynic peptide from a cerebral tissue of chicken that get easily. Two anti-allodynic substances were isolated from 800 chicken brains and named AIS (allodynia inhibitory substance)1 and AIS2, respectively. AIS1 determined the molecular weight as a result of the mass spectrometric analysis, but it is not determined come to structural determination.

Academic Significance and Societal Importance of the Research Achievements

本研究でニワトリ脳組織より単離精製した抗アロディニア活性物質AIS1、AIS2は非常に微量で有りながら神経因性疼痛に対して強い抑制活性を示す。神経因性疼痛においては、既存の鎮痛薬の効果が乏しく、新規の鎮痛薬の開発が急務となっている。AIS1、2の構造を明らかにすることができれば、合成物を作り、生理機能や作用機序を詳細に研究することによって、新たな神経因性疼痛治療薬の開発につながることが期待できる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016 2015

All Presentation (5 results)

  • [Presentation] Antiallodynic activity of APGWamide appears in the same manner as that activity of antidepressant, milnacipran2018

    • Author(s)
      Tetsuya Ikeda, Ryuichiro Takeda and Yasushi Ishida
    • Organizer
      日本比較生理生化学会 第40回神戸大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 軟体動物由来の神経ペプチド,APGWamide,の神経因性疼痛ラットに対する鎮痛効果2017

    • Author(s)
      池田哲也, 神谷莉香, 武田龍一郎, 石田康
    • Organizer
      日本動物学会 第88回 富山大会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] APGWamide analogue peptides showed potent antiallodynic activity in the rats with diabetic neuropathy2016

    • Author(s)
      Tetsuya Ikeda, Rika Kamiya, Ryuichiro Takeda and Yasushi Ishida
    • Organizer
      第40回日本比較内分泌学会・第37回日本比較生理生化学会 合同大会
    • Place of Presentation
      広島アステールプラザ(広島県広島市)
    • Year and Date
      2016-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 糖尿病ラットにおける熱アロディニアに対するAPGWamideの抑制効果2016

    • Author(s)
      池田哲也,神谷莉香,武田龍一郎,石田康
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 糖尿病ラットに対するAPGWamideアナログペプチドの抗アロディニア効果2015

    • Author(s)
      神谷莉香,瀧本真由美,武田龍一郎,石田康,池田哲也
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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