| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In order to establish projection neuron-specific calcium imaging in the spinal dorsal horn, I performed birthdate and lineage analyses of these neurons and aimed at the identification of their specific markers. Birthdate analysis using EdU revealed that most projection neurons are born between E9.5 and E10.5 in the mouse. In the developing dorsal neural tube, six classes of neuronal populations (dI1-dI6) are born from distinct progenitor domains around E10. Further analysis using reporter mice labeling Lbx1 lineage neurons (dI4-dI6) and histochemistry demonstrated that most projection neurons are derived from the dI5 progenitor domain.
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