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Establishment of pain evaluation system by using projection neuron-specific calcium imaging in the spinal dorsal horn

Research Project

Project/Area Number 15K08683
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pain science
Research InstitutionKansai Medical University

Principal Investigator

NISHIDA Kazuhiko  関西医科大学, 医学部, 助教 (80448026)

Co-Investigator(Kenkyū-buntansha) 松村 伸治  関西医科大学, 医学部, 准教授 (70276393)
伊藤 誠二  関西医科大学, 医学部, 教授 (80201325)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords脊髄後角 / 疼痛 / 投射ニューロン / カルシウムイメージング
Outline of Final Research Achievements

In order to establish projection neuron-specific calcium imaging in the spinal dorsal horn, I performed birthdate and lineage analyses of these neurons and aimed at the identification of their specific markers. Birthdate analysis using EdU revealed that most projection neurons are born between E9.5 and E10.5 in the mouse. In the developing dorsal neural tube, six classes of neuronal populations (dI1-dI6) are born from distinct progenitor domains around E10. Further analysis using reporter mice labeling Lbx1 lineage neurons (dI4-dI6) and histochemistry demonstrated that most projection neurons are derived from the dI5 progenitor domain.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Developmental origin of long-range neurons in the superficial dorsal spinal cord.2017

    • Author(s)
      Nishida, K., and Ito, S.
    • Journal Title

      European Journal of Neuroscience

      Volume: 46 Issue: 10 Pages: 2608-2619

    • DOI

      10.1111/ejn.13736

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal circuitry for sensory processing in the spinal dorsal horn2016

    • Author(s)
      Nishida K.
    • Journal Title

      生化学

      Volume: 88 Issue: 2 Pages: 229-232

    • DOI

      10.14952/SEIKAGAKU.2016.880229

    • NAID

      40020843401

    • ISSN
      0037-1017
    • Year and Date
      2016-04-25
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vivo two-photon imaging of structural dynamics in the spinal dorsal horn in an inflammatory pain model.2015

    • Author(s)
      Matsumura S, Taniguchi W, Nishida K, Nakatsuka T, Ito S
    • Journal Title

      European Journal of Neuroscience

      Volume: 41 Issue: 7 Pages: 987-995

    • DOI

      10.1111/ejn.12837

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Developmental origin of long-range projection neurons in the spinal dorsal horn2017

    • Author(s)
      西田和彦、伊藤誠二
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Chasing morphological changes of neuronal processes in the spinal dorsal horn in an inflammatory pain model by using two-photon microscopy2015

    • Author(s)
      Matsumura S., Taniguchi W., Nishida K., Nakatsuka T., Ito S.
    • Organizer
      北米神経科学会2015
    • Place of Presentation
      シカゴ、アメリカ
    • Year and Date
      2015-10-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 分子脳科学2015

    • Author(s)
      西田和彦、伊藤誠二他、(三品昌美編)
    • Total Pages
      312
    • Publisher
      化学同人
    • Related Report
      2015 Research-status Report
  • [Remarks] 関西医科大学 医化学講座

    • URL

      http://www3.kmu.ac.jp/medchem/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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