Investigation of the role of prostanoids in pain maintenance using in vivo imaging

• Project/Area Number: 15K08684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Kansai Medical University
• Principal Investigator: MATSUMURA Shinji 関西医科大学, 医学部, 准教授 (70276393)
• Co-Investigator(Kenkyū-buntansha): 伊藤 誠二 関西医科大学, 医学部, 教授 (80201325) ; 西田 和彦 関西医科大学, 医学部, 助教 (80448026)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 疼痛 / 神経障害性疼痛 / 多光子励起顕微鏡 / プロスタグランジンE2 / 疼痛の神経機構 / プロスタグランジン / バイオイメージング / プロスタグランジンＥ２ / 脊髄後角ニューロン

Outline of Final Research Achievements

One of the features of neuropathic pain is that tactile stimulation is perceived as pain sensation. We attempted to visualize the plasticity mechanism that causes this modulation as a morphological and functional change in the spinal cord neuronal network. We continuously tracked morphological changes of neuronal structures and recorded calcium ion dynamics in neuronal somata associated with them. Intracellular calcium imaging of multiple spinal dorsal horn neurons was performed in two-photon microscopy on in vivo preparations of spontaneous-respiratory individuals. The response was recorded by PGE2 in neurons that increase intracellular calcium ion concentration in response to mechanical stimulation to the skin. The neuron was present on the dorsal horn surface of the spinal cord.

Research Products

• [Journal Article] Impaired peripheral nerve regeneration in type-2 diabetic mouse model. (2018)
  • Author(s): Pham M. V., Nguyen H. T., Katano, T., Matsumura, S., Aaito, S., Yamada A., Furue, H. and Ito, S.
  • Journal Title: European Journal of Neuroscience Volume: 47 Issue: 2 Pages: 126-139
  • DOI: 10.1111/ejn.13771
  • Peer Reviewed
• [Journal Article] Na+/K+-ATPase coupled to endothelin receptor type B stimulates peripheral nerve regeneration via lactate signaling. (2017)
  • Author(s): Nguyen H. T., Katano, T., Matsumura, S., Funatsu, N., Pham, M. V., Fujisawa, JI. and Ito, S.
  • Journal Title: European Journal of Neuroscience Volume: 46 Issue: 5 Pages: 2096-2107
  • DOI: 10.1111/ejn.13647
  • Peer Reviewed
• [Journal Article] RNA editing enzyme ADAR2 is a mediator of neuropathic pain after peripheral nerve injury. (2017)
  • Author(s): Uchida H, Matsumura S, Okada S, Suzuki T, Minami T and Ito S
  • Journal Title: FASEB Journal Volume: 印刷中 Issue: 5 Pages: 1847-1855
  • DOI: 10.1096/fj.201600950r
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 神経細胞特異的に蛍光タンパクを発現するマウスを用いた坐骨神経切断-再生モデルの確立と神経再生女神ズムの解析 (2017)
  • Author(s): 伊藤 誠二、Tu Nguyen H、Pham Vuong H、片野 泰代、松村 伸治
  • Journal Title: 麻酔 Volume: 66
• [Journal Article] Role of c-Jun N-terminal kinase in late nerve regeneration monitored by in vivo imaging of thy1-yellow fluorescent protein transgenic mice. (2016)
  • Author(s): Nguyen H. T., Katano, T., Matsumura, S., Pham M. V., Muratani, T., Minami, T. and Seiji Ito.
  • Journal Title: Eur. J. Neurosci. Volume: 43 Issue: 4 Pages: 548-560
  • DOI: 10.1111/ejn.13139
  • Peer Reviewed / Open Access
• [Journal Article] In vivo two-photon imaging of structural dynamics in the spinal dorsal horn in an inflammatory pain model. (2015)
  • Author(s): Matsumura S, Taniguchi W, Nishida K, Nakatsuka T, Ito S
  • Journal Title: European Journal of Neuroscience Volume: 41 Issue: 7 Pages: 987-995
  • DOI: 10.1111/ejn.12837
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of mechanisms of allodynia induced by acromelic acid A between early and late phases. (2015)
  • Author(s): Omoto H, Matsumura S, Kitano M, Miyazaki S, Minami T, Ito S.
  • Journal Title: European Journal of Pharmacology Volume: In Press Pages: 42-48
  • DOI: 10.1016/j.ejphar.2015.03.075
  • Peer Reviewed
• [Presentation] Na+/K+-ATPase coupled to ETBRpromotes peripheral nerve regeneration via lactate signaling. (2017)
  • Author(s): Ito S, Nguyen TH, Katano T, Matsumura S, Nomuo F, Pham VM.
  • Organizer: The 47th Annual Meeting of the Society for Neuroscience (Washington, U.S.A.)
  • Int'l Joint Research
• [Presentation] Impaired peripheral nerve regeneration in type 2 diabetic mouse model. (2017)
  • Author(s): Pham VM, Nguyen TH, Katano T, Matsumura S, Ito S.
  • Organizer: The 47th Annual Meeting of the Society for Neuroscience (Washington, U.S.A.)
  • Int'l Joint Research
• [Presentation] Involvement of Na+/K+-ATPase in peripheral nerve regeneration via lactate signaling in sciatic nerve transection-regeneration model (2016)
  • Author(s): Tu NH, Ito S, Matsumura S, Katano T, Pham VM
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, 米国
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Involvement of c-jun N-terminal kinase in neurite extension of cultured DRG neurons (2015)
  • Author(s): Nguyen HT, Matsumura S, Katano T, Ito S
  • Organizer: Society for Neuroscience
  • Place of Presentation: 米国 シアトル
  • Year and Date: 2015-10-19
  • Int'l Joint Research
• [Presentation] Chasing morphological changes of neuronal processes in the spinal dorsal horn in an inflammatory pain model by using two-photon microscopy (2015)
  • Author(s): Matsumura S, Taniguchi W, Nishida K, Nakatsuka T, Ito S.
  • Organizer: Society for Neuroscience
  • Place of Presentation: 米国 シアトル
  • Year and Date: 2015-10-18
  • Int'l Joint Research