Elucidation of the drug-resistance acquisition mechanism in Mycobacterium tuberculosis to be multidrug and extremely-multidrug resistant and identification of those possible genetic markers.
Project/Area Number |
15K08724
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Epidemiology and preventive medicine
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Research Institution | Hokkaido University |
Principal Investigator |
Nakajima Chie 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (60435964)
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Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Yasuhiko 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90206540)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 結核 / 多剤耐性 / 遺伝子解析 / 型別 / 疫学 / M. tuberculosis / 多剤耐性菌 / 全ゲノム解析 |
Outline of Final Research Achievements |
Sequential acquisition of mutations in drug-resistance associating genes causes an emergence of multidrug-resistant Mycobacterium tuberculosis (MDR-MTB). The Beijing genotype of MTB is known to be associating with MDR, and its prevalence among MDR-MTB population was significantly higher than that in non-MDR MTB in three TB high-burden countries; Myanmar, Nepal and Thailand. The majority of the Beijing type isolates belonged to the “modern” subtype and most of them had putative KatG S315T and RpsL K43R mutations suggesting those were isoniazid and streptomycin resistant. Those genetic characteristics can be used as a marker of transmissible MDR-MTB; thus, we have developed simple detection methods including a multiplex PCR to identify the “modern” type of the Beijing lineage and a line probe assay to diagnose isoniazid resistance.
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Report
(4 results)
Research Products
(41 results)
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[Presentation] Diverse molecular genotypes of Mycobacterium tuberculosis isolates circulating in Kandy, Sri Lanka2018
Author(s)
C MENDIS, C RATNATUNGA, V THEVANESAM, A KUMARA, S WICKRAMASINGHE, D MADEGEDARA, C GAMAGE, C NAKAJIMA, Y SUZUKI
Organizer
U.S.-Japan Cooperation Medical Science Program, 54nd Mycobacteria Panel Meeting
Related Report
Int'l Joint Research
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[Presentation] MOLECULAR EPIDEMIOLOGY OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN ASIAN COUNTRIES2016
Author(s)
Chie Nakajima, Bhagwan Maharjan, Khin Saw Aye, Zeaur Rahim, Benjawan Phetsuksiri, Aki Tamaru, Nan Aye Thida Oo, Lai Lai San, Yogendra Shah, Janisara Rudeeaneksin, Jeewan Thapa, Ajay Poudel and Yasuhiko Suzuki
Organizer
US-Japan CMSP 50th Anniversary TB, Leprosy Panel Meeting
Place of Presentation
Bethesda North Marriott Hotel & Conference Center (U.S.A.)
Year and Date
2016-01-13
Related Report
Int'l Joint Research
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[Presentation] MIRU-VNTR Typing of Beijing MDR-TB strains from Myanmar2016
Author(s)
Lai Lai San, Nan Aye Thida Oo, Wah Wah Aung, Khin Saw Aye, Chie Nakajima, Yasuhiko Suzuki
Organizer
US-Japan CMSP 50th Anniversary TB, Leprosy Panel Meeting
Place of Presentation
Bethesda North Marriott Hotel & Conference Center (U.S.A.)
Year and Date
2016-01-13
Related Report
Int'l Joint Research
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[Presentation] Genetic Diversity and Transmission Dynamics of Multidrug Resistant Tuberculosis in Nepal2016
Author(s)
Bhagwan Maharjan, Yasuhiko Suzuki, Chie Nakajima, Ajay Poudel, Harald Hoffmann, Korkut Avsar, Bhabana Shrestha, Jeewan Thapa, Yogendra Shah, Rajendra Pant
Organizer
US-Japan CMSP 50th Anniversary TB, Leprosy Panel Meeting
Place of Presentation
Bethesda North Marriott Hotel & Conference Center (U.S.A.)
Year and Date
2016-01-13
Related Report
Int'l Joint Research
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[Presentation] Molecular characteristics and genotypes of Mycobacterium tuberculosis isolated from an outbreak of Thailand-Myanmar border2016
Author(s)
Janisara Rudeeaneksin, Benjawan Phetsuksiri, Supranee Bunchoo, Sopa Srisungngam, Wiphat Klayut, Somchai Sangkitporn, Anupong Sujariyakul, Krairerk Suthum, Nattakan Tipkrual, Yukari Fukushima3 Haruka Suzuki, Chie Nakajima, Yasuhiko Suzuki
Organizer
US-Japan CMSP 50th Anniversary TB, Leprosy Panel Meeting
Place of Presentation
Bethesda North Marriott Hotel & Conference Center (U.S.A.)
Year and Date
2016-01-13
Related Report
Int'l Joint Research