| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The drug resistance rate was estimated to be 9.2% among the 2,629 referred samples from patients receiving antiretroviral treatment in 2015-2016 in the Philippines. Thirty-seven HIV strains were isolated from 142 HIV-positive serum/plasma samples of patients harboring drug-resistant strains with detectable viral load after a 12-month treatment of drug-naive patients. The replication competence was then assessed by re-inoculation into seronegative peripheral blood mononuclear cells in vitro. The viral yield correlated with the plasma viral load. However, more progeny viruses were produced after the in vitro re-inoculation than the corresponding plasma viral load. Intrinsic replication competence could be assessed by the in vitro re-inoculation system, but not be estimated from in vivo plasma viral load. These results suggest that the replication competence of HIV become an important marker especially when a drug regimen should be altered.
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