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Investigation of the initial immune-responsiveness and memory immunity to the hepatitis B vaccine

Research Project

Project/Area Number 15K08746
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Epidemiology and preventive medicine
Research InstitutionIwate Medical University

Principal Investigator

Miyasaka Akio  岩手医科大学, 医学部, 准教授 (80382597)

Research Collaborator Takikawa Yasuhiro  
Wang Tang  
Yoshida Yuichi  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsHBワクチン / TCR β レパトア / BCR IgG H chain レパトア / TCRβレパトア / BCR IgG H chainレパトア
Outline of Final Research Achievements

To clarify an immune response for the hepatitis B (HB) vaccine, we conducted high-throughput sequencing of the T cell receptor (TCR) beta chain and B cell receptor (BCR) IgG heavy (H) chain complementary determining region 3 (CDR3) repertoires in 5 volunteers before and after the immunizations with the HB vaccine. The all 5 participants acquired HBs antibody. The TCR beta chain CDR3 repertoire diversity significantly increased, while the BCR IgG H chain CDR3 repertoire diversity significantly decreased after the second vaccination. These diversity changes might be associated with a better response to the HB vaccine. It is possible that the TCR beta chain and BCR IgG H chain CDR3 repertoires may have changed within the same numbers of unique reads. Our data failed to identify the specific dominant clones that responded to the HB vaccine.
As for our investigation of the memory immunity, the HB-vaccinated subjects had very low numbers of memory B cells in their peripheral blood.

Academic Significance and Societal Importance of the Research Achievements

HBワクチンに対する初期免疫応答について解析を行ない、HBs抗体獲得者において2回接種後のTCRβ chainレパトア、BCR IgG H chainレパトアの変化はHBs抗体獲得のサロゲートマーカーとなる可能性が示唆された。今後、さらに解析をすすめることにより不応例への対策やワクチン回数を減らすための方策などに役立つと考えられた。
また、HBワクチン接種歴ある者ではmemory B細胞は末梢血中に僅かに存在している可能性があった。HBワクチンのユニバーサルワクチネーションが施行されるようになりHBs抗体獲得後の免疫記憶の有効性を明らかにすることは追加接種を減らすなどの有用性があると考えられた。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2019 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination2019

    • Author(s)
      Miyasaka Akio、Yoshida Yuichi、Wang Ting、Takikawa Yasuhiro
    • Journal Title

      Human Vaccines & Immunotherapeutics

      Volume: 印刷中 Issue: 11 Pages: 2738-2753

    • DOI

      10.1080/21645515.2019.1600987

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of amino acids in antigen-binding site of class II HLA proteins independently associated with hepatitis B vaccine response2017

    • Author(s)
      Aiko Sakai, Emiko Noguchi, Takashi Fukushima, Manabu Tagawa, Atsushi Iwabuchi, Masaki Kita, Keisuke Kakisaka, Akio Miyasaka, Yasushiro Takikawa, Ryo Sumazaki
    • Journal Title

      Vaccine

      Volume: 35 Pages: 703-710

    • NAID

      120007135005

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 次世代シークエンサーを用いたHBVワクチン接種前後の末梢血中における免疫多様性の検討2017

    • Author(s)
      宮坂昭生、吉田雄一、滝沢康裕
    • Organizer
      第21回日本ワクチン学会学術集会
    • Related Report
      2017 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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