Characterization of Novel Staphylococcal Chromosomal Cassette (SCC) Composite Islands in MRSA clinical isolates, and application to molecular epidemiology

• Project/Area Number: 15K08781
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Sapporo Medical University
• Principal Investigator: Urushibara Noriko 札幌医科大学, 医学部, 准教授 (80396308)
• Co-Investigator(Kenkyū-buntansha): 小林 宣道 札幌医科大学, 医学部, 教授 (80186759)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ブドウ球菌 / MRSA / SCCmec / 水平伝播 / アルギニン代謝 / ポリアミン / コアグラーゼ陰性ブドウ球菌 / コアグラーゼ陰性ブドウ球菌ゲノム / SCC / ACME / メチシリン耐性 / ゲノム / 薬剤耐性

Outline of Final Research Achievements

The staphylococcal cassette chromosome (SCC) is a bacterial mobile genetic element inserted in the genome of staphylococcal species. SCC contains a variety of functional genes that provide biological benefits to the host bacteria. Staphylococcal species exchange genetic information by horizontal gene transfer of SCCs, which enables rapid adaption to changing environments. Three ST5-MRSA strains (SC640, SC792, and SC955) positive for both ACME-arc and speG were subjected to whole-genome sequencing, and their SCC composite islands (SCC-CIs) were determined. The SCC-CIs of these strains contained following novel SCCs: novel SCCs containing speG gene (SC640, SC792, and SC955), a novel SCCmec type XIV (SC792), and a truncated SCCmec type XIV-related structure (SC640).

Academic Significance and Societal Importance of the Research Achievements

薬剤耐性菌の蔓延は世界的な問題である。新規の抗生剤の開発が停滞している現状では，耐性菌の動向調査に基づく感染制御・投薬管理が必要不可欠である。本研究ではブドウ球菌の可動性遺伝エレメント SCC の構造に着目し，遺伝子再編が現在も進行であり，更に複雑化・多様化していることを明らかにした。分子疫学解析を行う際の基盤となる遺伝情報を拡充し，分子多型に基づく耐性菌監視の改善に資する結果と言える。他方，再編に伴い宿主細菌が新たな生物学優位性を持つことも予測され，継続的な動向調査の必要性を示すことができた。

Research Products

• [Journal Article] Molecular epidemiological characterization of methicillin-susceptible and -resistant Staphylococcus aureus isolated from skin and soft tissue infections in Bangladesh. (2019)
  • Author(s): Haque N, Aung MS, Paul SK, Bari MS, Ahmed S, Sarkar SR, Roy S, Nasreen SA, Mahmud MC, Hossain MA, Urushibara N, Kawaguchiya M, Sumi A, Kobayash N
  • Journal Title: Microbial Drug Resistance Volume: 25 Issue: 2 Pages: 241-250
  • DOI: 10.1089/mdr.2018.0123
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar. (2017)
  • Author(s): Aung MS, San T, Aye MM, Mya S, Maw WW, Zan KN, Htut WHW, Kawaguchiya M, Urushibara N, Kobayashi N
  • Journal Title: Toxins Volume: 9 Issue: 8 Pages: 241-253
  • DOI: 10.3390/toxins9080241
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) from outpatients in northern Japan : increasing tendency of ST5/ST764 MRSA-IIa with arginine catabolic mobile element (ACME II’) (2017)
  • Author(s): Aung MS, Kawaguchiya M, Urushibara N, Sumi A, Ito M, Kudo K, Morimoto S, Hosoya S, Kobayashi N
  • Journal Title: Microbial Drug Resistance Volume: 印刷中 Issue: 5 Pages: 616-625
  • DOI: 10.1089/mdr.2016.0176
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Drug resistance and genetic characteristics of clinical isolates of staphylococci in Myanmar : high prevalence of PVL among methicillin-susceptible Staphylococcus aureus belonging to various sequence types. (2016)
  • Author(s): Aung MS, Zi H, New KM, Maw WW, Aung MT, Min WW, Nyein N, Kawaguchiya M, Urushibara N, Sumi A, Kobayashi N
  • Journal Title: New Microbes and New Infections Volume: 10 Pages: 58-65
  • DOI: 10.1016/j.nmni.2015.12.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Arginine catabolic mobile elements in methicillin-resistant Staphylococcus aureus (MRSA) genotype ST5-MRSA-II and ST764-MRSA-II in Japan: novel structures and temporal changes. (2016)
  • Author(s): Urushibara N, Kawaguchia M, Onishi M, Mise K, Aung MS, Kobayashi N.
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 60 Issue: 5 Pages: 3119-3122
  • DOI: 10.1128/aac.02356-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Detection of ST772 Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus (Bengal Bay clone) and ST22 S. aureus isolates with a genetic variant of elastin binding protein in Nepal. (2016)
  • Author(s): Pokhrel RM , Aung MS, Thapa B, Chaudhary R, Mishra SK, Kawaguchiya M, Urushibara N, Kobayashi N.
  • Journal Title: New Microbes and New Infections Volume: 11 Pages: 20-27
  • DOI: 10.1016/j.nmni.2016.02.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 北海道にて分離された MRSA に見いだされた新規 SCCmec-SCC 複合体 (2019)
  • Author(s): 漆原範子、アウン・メイジ・ソウ、川口谷充代、小林宣道
  • Organizer: 第89回日本衛生学会学術総会
• [Presentation] Molecular epidemiological study of MRSA harbouring ACME (arginine catabolic mobile element) in Hokkaido, Japan (2017)
  • Author(s): 小林宣道，メイジ・ソウアウン，川口谷充代，漆原範子．
  • Organizer: 第 87 回日本衛生学会
  • Place of Presentation: フェニックスシーガイアリゾート（宮崎市）
  • Year and Date: 2017-03-26
• [Presentation] ミャンマーにおける食品取扱い者由来の黄色ブドウ球菌およびアルジェンテウスブドウ球菌の遺伝子型と病原因子 (2017)
  • Author(s): メイジ・ソウアウン，サン・ティダ，川口谷充代，漆原範子，小林宣道．
  • Organizer: 第 90 回日本細菌学会
  • Place of Presentation: 仙台国際センター展示棟（仙台市）
  • Year and Date: 2017-03-18
• [Presentation] Variation in ACME-SCCmec composite elements in MRSA clinical isolates in the CC5 lineage in Japan (2017)
  • Author(s): Urushibara N, Aung MS, Kawaguchiya M, Kobayashi N
  • Organizer: International Union of Microbiological Societies Congress 2017
  • Int'l Joint Research
• [Presentation] Molecular Characterization of Methicillin-Resistant Staphylococcus Aureus (MRSA) from Outpatients in Hokkaido, Japan (2017)
  • Author(s): Aung MS, Kawaguchiya M, Urushibara N, Kobayashi N
  • Organizer: International Union of Microbiological Societies Congress 2017
  • Int'l Joint Research
• [Presentation] 本邦での黄色ブドウ球菌ゲノム中のACME（アルギニン代謝系可動性遺伝子要素） (2017)
  • Author(s): 漆原範子，川口谷充代，メイジ・ソウ・アウン，小林 宣道
  • Organizer: 第６２回日本ブドウ球菌研究会
• [Presentation] Molecular epidemiology of MRSA isolates harbouring PVL genes and/or ACME (arginine catabolic mobile element) in Hokkaido (2017)
  • Author(s): メイジ・ソウ・アウン，漆原範子，川口谷充代，小林宣道
  • Organizer: 第６２回日本ブドウ球菌研究会
• [Presentation] Molecular epidemiological characterizations of MRSA isolates harbouring PVL genes and/or ACME (arginine catabolic mobile element) in Hokkaido, northern main island of Japan. (2016)
  • Author(s): Urushibara N, Aung MS, Kawaguchiya M, Kobayashi N
  • Organizer: The 16th Asia Pacific Congress of Clinical Microbiology and Infection
  • Place of Presentation: The Melbourne Convention and Exhibition Centre（メルボルン，オーストラリア）
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] Panton-valentine leukoidin (PVL)/ Arginine catabolid mobile element (ACME)-positive methicilline-resistant Staphylococcus aureus (MRSA) in the community and hospital in Japan. (2016)
  • Author(s): Kawaguchiya M, Urushibara N, Aung MS, Kobayashi N.
  • Organizer: The 17th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Nine Tree Convention（ソウル，韓国）
  • Year and Date: 2016-08-30
  • Int'l Joint Research
• [Presentation] バングラデシュにおける産褥期感染症由来の大腸菌と黄色ブドウ球菌の分子疫学的解析 (2016)
  • Author(s): 小林宣道，アーメッド・サルマ，川口谷充代，漆原範子，メイジ・ソウアウン．鷲見紋子．
  • Organizer: 第 86 回日本衛生学会
  • Place of Presentation: 旭川市民文化会館（旭川市）
  • Year and Date: 2016-05-11
• [Presentation] Genetic characterization of hospital-acquired Staphylococcal aureus in Nepal (2016)
  • Author(s): Kobayashi N, Aung M, Kawaguchiya M, Urushibara N
  • Organizer: 第 89 回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪市）
  • Year and Date: 2016-03-23