Simultaneous analysis of highly polar pharmaceutical adulterants in slimming products by hydrophilic interaction liquid chromatography
| Project/Area Number |
15K08818
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | Iwaki Meisei University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アルカロイド / 固相抽出 / エフェドリン / シネフェリン / 蛍光誘導体化試薬 / シネフリン / NBD-F / 固相抽出法 |
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| Outline of Final Research Achievements |
Analysis methods of atropine and scopolamine, which are tropane alkaloids to be included in gastrointestinal medicine, in particular, gastrointestinal medicine, were examined by solid phase extraction using cartridges having both characteristics of strong cation exchange and reversed phase, and good analysis was conducted It has become possible.
Highly sensitive detection method for coloring alkaloids in crude drug ingredients, which is difficult to analyze, with NBD - F was studied. We examined using HPLC and detector with ultraviolet-visible absorption spectrophotometer / fluorometer. The alkaloid-containing sample extract was developed with NBD-F reagent and the fluorescence was further measured. Quantitative sensitivity of three kinds of ephedrine was about 10 times better than that of the conventional method.
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Report
(4 results)
Research Products
(3 results)
