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Study on establishment of forensic genetic analysis of degraded DNA samples and improvement of accuracy in the judgment

Research Project

Project/Area Number 15K08868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionShinshu University

Principal Investigator

ASAMURA Hideki  信州大学, 学術研究院医学系, 教授 (80324250)

Co-Investigator(Kenkyū-buntansha) 佐藤 紀子  信州大学, 学術研究院医学系, 助手 (00649254)
塩崎 哲也  信州大学, 医学部, 助教(特定雇用) (00722018)
亀井 佐矢子  信州大学, 医学部, 特任助教 (10735153)
林 徳多郎  信州大学, 学術研究院医学系, 助教 (50600607)
布谷 美弥  信州大学, 医学部, 特任助教 (10759921)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsDNA多型 / INDEL / 劣化DNA / 個人識別 / 劣化DNA試料 / INDEL多型
Outline of Final Research Achievements

The objective of this research is to improve accuracy in analyzing degraded DNA samples. We researched Japanese population genetic data of 19 insertion-deletion polymorphisms and 11 X-STRs in X chromosome using newly developed multiplex PCR systems. We investigated suitable parts for DNA analysis from hard tissues and researched the optimal conditions of decalcification in DNA extraction from skeletal remains.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] 硬組織試料のDNA抽出法―脱灰処理条件と抽出効率の比較検討―.2017

    • Author(s)
      永井みどり,佐藤紀子,塩崎哲也,浅村英樹.
    • Journal Title

      DNA多型

      Volume: 25 Pages: 123-126

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 11 X-STRs mulutiplexシステム及び19INDELs フラグメント解析システムによる日本人集団遺伝子解析.2017

    • Author(s)
      佐藤紀子,塩崎哲也,永井みどり,太田正穂,浅村英樹.
    • Journal Title

      DNA多型

      Volume: 25 Pages: 161-163

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Multiple Assays using 46 SNPs for Comprehensive mtDNA Haplogrouping and Application to Highly Degraded DNA.2016

    • Author(s)
      Nunotani M, Sato N, Kamei S, Shiozaki T, Takatsu K, Hayashi T, Asamura H.
    • Journal Title

      J Forensic Sci

      Volume: 61 Issue: 2 Pages: 472-477

    • DOI

      10.1111/1556-4029.12961

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 10 X-STRs multiplex systemを用いた日本人集団遺伝子解析.2016

    • Author(s)
      布谷美弥,佐藤紀子,永井みどり,塩崎哲也,林德多郎,浅村英樹.
    • Journal Title

      DNA多型

      Volume: 24 Pages: 229-231

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of 30 insertion-deletion polymorphisms in the Japanese population using th e Investigator DIPplex kit.2015

    • Author(s)
      Nunotani M, Shiozaki T, Sato N, Kamei S, Takatsu K, Hayashi T, Ota M, Asamura H
    • Journal Title

      . Legal Medicine

      Volume: 17 Issue: 6 Pages: 467-470

    • DOI

      10.1016/j.legalmed.2015.10.002

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 硬組織試料のDNA抽出法―部位によるDNA抽出量の比較検討―2017

    • Author(s)
      佐藤紀子,塩崎哲也,浅村英樹.
    • Organizer
      第26回日本DNA多型学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 硬組織試料のDNA抽出法―脱灰処理条件と抽出効率の比較検討―2016

    • Author(s)
      永井みどり,佐藤紀子,塩崎哲也,浅村英樹.
    • Organizer
      第25回日本DNA多型学会学術集会
    • Place of Presentation
      千葉(東京大学 大気海洋研究所)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 11 X-STRs mulutiplexシステム及び19INDELs フラグメント解析システムによる日本人集団遺伝子解析.2016

    • Author(s)
      佐藤紀子,塩崎哲也,永井みどり,太田正穂,浅村英樹.
    • Organizer
      第25回日本DNA多型学会学術集会
    • Place of Presentation
      千葉(東京大学 大気海洋研究所)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 硬組織試料のDNA抽出法の検討―試料の陳旧度に応じた方法の確立―2016

    • Author(s)
      永井みどり,佐藤紀子,布谷美弥,塩崎哲也,林德多郎,太田正穂,浅村英樹.
    • Organizer
      第100次日本法医学会学術全国集会
    • Place of Presentation
      東京(きゅりあん)
    • Year and Date
      2016-06-15
    • Related Report
      2016 Research-status Report
  • [Presentation] INDEL多型領域を用いたフラグメント解析システムの検討2015

    • Author(s)
      佐藤 紀子, 布谷 美弥, 亀井佐矢子,塩崎 哲也,林 德多郎,太田 正穂,浅村 英樹.
    • Organizer
      第99次日本法医学会学術全国集会
    • Place of Presentation
      高知市文化プラザ
    • Year and Date
      2015-06-11
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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